Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.7.7 (
DNA polymerase
)
17,007
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The usefulness of gold compounds in the therapy of rheumatoid arthritis is well established, however, the pharmacological mechanisms of the compounds are still unclear. In this report, effects of gold compounds on DNA synthesis were examined.
Gold sodium thiomalate
inhibited DNA synthesis in the HeLa "nuclei system" as well as in the enzyme reaction using
DNA polymerase alpha
. More precisely, gold sodium thiomalate inhibited the activity of
DNA polymerase alpha
using activated DNA, poly[d(A-T)] or poly[d(G-C)] for the template, but did not inhibit the activity of
DNA polymerase I
with each template. The compound had also no inhibitory effect on
DNA polymerase beta
or gamma. On the other hand, auranofin inhibited the incorporation of [3H]thymidine into HeLa DNA but did not inhibit DNA synthesis in the HeLa "nuclei system". The inhibition of
DNA polymerase alpha
activity by gold sodium thiomalate was competitive with poly(dA).oligo(dT) for template but noncompetitive with dTTP. Thus, gold sodium thiomalate is a potent and specific inhibitor of
DNA polymerase alpha
and this inhibitory effect could play an important role in the therapeutic and pharmacological effects of gold sodium thiomalate.
...
PMID:Inhibitory effects of gold sodium thiomalate on DNA polymerase alpha. 251 17
