Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.7.7 (
DNA polymerase
)
17,007
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
We measured the levels of the DNA polymerases alpha, beta, and gamma in human peripheral lymphocyte cells stimulated with
Tora
-mame lectin (TM-lectin) and the induction patterns were compared to those with other plant lectins, i.e., phytohemagglutinin (PHA) and pokeweed mitogen (PWM). The maximum activity of
DNA polymerase alpha
in lymphocytes was achieved at the concentration of 10 micrograms/ml with TM lectin and the dose response curve of TM lectin showed a sharp peak in contrast to that of PWM. During prolonged stimulation for 10 days, the time course of
DNA polymerase alpha
induction was different among these three lectins. A peak of alpha-enzyme was correlated with maximal incorporation of [3H]thymidine and was observed on the fourth day with TM lectin, on the third day with PHA, and sixth day with PWM.
DNA polymerase beta
in lymphocytes was also activated by the addition of these proteins. Two different peaks were observed during a 10-day period with every lectin, and TM lectin was most potent stimulator among them. The activity of
DNA polymerase gamma
in lymphocytes was at a very low but detectable level which increased slightly in response to TM lectin treatment. Although some variability of gamma-enzyme activity was observed after the seventh day, the pattern in the course of 7 days was similar among the lectins.
...
PMID:DNA polymerase alpha, beta, and gamma activities in human lymphocytes stimulated by Tora-mame (Phaseolus vulgaris) lectin. 129 Apr 61
