Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:2.7.7.7 (DNA polymerase)
 17,007 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In recent years, human papillomavirus (HPV) infection in female genital organs has attracted increasing attention because of its association with lesions in the uterine cervix, especially cervical carcinoma. In this study, the author attempted to determine the presence or absence of HPV infection in the cervical region by means of the polymerase chain reaction (PCR) method, which has become practical thanks to the development of thermostable DNA polymerase, and compared this method with traditional Southern blotting. Moreover, we conducted PCR after detecting DNA by two methods: target punch biopsy and cervicovaginal lavage, and compared the results in terms of detection. 1. The results of HPV detection from the isolated tissue were compared with those of traditional Southern blotting and PCR combined with subsequent hybridization under high stringency (PCR-H). Among the cases that showed negative response with the former method, two of six specimens of cervical carcinoma tissue, and four of eight specimens of normal cervical tissue yielded a positive response as a result of hybridization under low stringency after PCR (PCR-L). HPV was investigated in 56 clinical specimens by PCR. PCR-L was positive in 50.0% and 20.0% of the specimens of cervical carcinoma and normal cervical tissue, respectively. With PCR-H, the positive rates were 37.5% and 7.5% in cervical carcinoma and normal cervical tissue, respectively. 2. Among CIN patients who were followed up at the outpatient clinic, PCR was conducted in specimens obtained by cervicovaginal lavage and target punch biopsy, and the rates of HPV detection were compared.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:[Detection of human papillomavirus DNA in uterine cervix by polymerase chain reaction (PCR) method]. 132 83

The distribution of DNA polymerase alpha-positive cells in neoplasia of the uterine cervix and in normal cervical epithelium was studied using a monoclonal antibody against DNA polymerase alpha. The positive cells were found only in the parabasal layer of normal cervical epithelium and only in the nonkeratinized areas of the cancer nests of invasive keratinizing carcinoma. Most cells in cancer nests of an invasive nonkeratinizing carcinoma were found to be DNA polymerase alpha-positive. In cases of mild or moderate dysplasia DNA polymerase alpha-positive cells were found only in the lower half of the epithelium. DNA polymerase alpha-positive cells in severe dysplasia to carcinoma in situ were distributed throughout the full thickness of the epithelium. The percentages of DNA polymerase alpha-positive cells in mild or moderate dysplasia, severe dysplasia to carcinoma in situ, and invasive carcinoma were 32.2%, 45.7%, and 53.7%, respectively. The authors previously developed immunohistochemical methods for detecting DNA polymerase alpha by monoclonal antibody that allowed the proliferative activity of cells in normal and neoplastic tissues to be estimated.
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PMID:Detection of proliferative cells in dysplasia, carcinoma in situ, and invasive carcinoma of the uterine cervix by monoclonal antibody against DNA polymerase alpha. 334 75

The polymerase chain reaction (PCR) was used to investigate samples from Indonesian and Swedish patients with cervical intraepithelial neoplasia grade III (CIN III), squamous cell carcinoma or adenocarcinoma of the cervix for the presence of a transforming fragment (BC 24) of herpes simplex virus type 2 (HSV-2) DNA. The PCR test for HSV-2 DNA was more sensitive than the infectivity endpoint titer in a cell culture system and no cross reactivity was found with either varicella-zoster virus, cytomegalovirus, Epstein-Barr virus, human papillomavirus 16 or 18, or human genomic DNA. Using this PCR test, 2 out of 5 cases with CIN III, 10 of 71 squamous cell carcinomas, and 3 of 11 adenocarcinomas of the uterine cervix were found to contain DNA sequences homologous to the BC 24 fragment of the HSV-2 genome. Only two of the samples containing this transforming region of the HSV-2 DNA were positive in a PCR assay for the HSV-2 DNA polymerase gene. The great majority of the HSV-2 BC 24 DNA positive (12 of 15) came from the Indonesian group of patients. All 15 CIN III or cancer samples positive for the HSV-2 BC 24 fragment were also positive for papillomavirus DNA. In line with observations made by others, our data support the hypothesis that HSV infection could represent one of several possible oncogenic cofactors leading to cervical carcinoma. The HSV cofactor might be more important in the Indonesian than in the Swedish population.
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PMID:Detection of the BC 24 transforming fragment of the herpes simplex virus type 2 (HSV-2) DNA in cervical carcinoma tissue by polymerase chain reaction (PCR). 779 6