Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.7.7 (
DNA polymerase
)
17,007
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
N
6
-Methyladenine (m
6
A) is the most abundant internal modification on mammalian mRNA. Very recently, m
6
A has been reported as a potentially important 'epigenetic' mark in eukaryotes. Until now, site-specific detection of m
6
A is technically very challenging. Here, we first reveal that m
6
A significantly hinders DNA- and RNA-directed DNA synthesis. Systematic investigations of 5'-triphosphates of a variety of 5-substituted 2'-deoxyuridine analogs in primer extension have been performed. In the current study, a quantitative analysis of m
6
A in the RNA or DNA context has been achieved, using
Bst
DNA polymerase
catalyzed primer extension. Molecular dynamics study predicted that m
6
A in template tends to enter into and be restrained in the
MGR
region of
Bst
DNA polymerase
, reducing conformational flexibility of the DNA backbone. More importantly, a site-specific determination of m
6
A in human ribosomal RNA (rRNA) with high accuracy has been afforded. Through a cumulative analysis of methylation alterations, we first reveal that significantly cancer-related changes in human rRNA methylation were present in patients with hepatocellular carcinoma.
...
PMID:
N
6
-Methyladenine hinders RNA- and DNA-directed DNA synthesis: application in human rRNA methylation analysis of clinical specimens. 2991 Sep 2
