Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.7.6 (
RNA polymerase
)
34,946
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
We report on an adult patient with de novo acute myeloid leukemia (AML) with a t(11;22)(q23;q11.2) involving
CDCREL1
and MLL genes. Reverse
transcriptase
(RT)-polymerase chain reaction (PCR) followed by direct sequencing analysis revealed the MLL-
CDCREL1
fusion transcript in his leukemic cells. Analysis of the fusion transcript showed that exon 6 of MLL was fused to exon 4 of
CDCREL1
, which contains an AT-hook domain of MLL and a GTP binding domain of
CDCREL1
. To investigate the roles of
CDCREL1
further, we examined the expression of the
CDCREL1
gene in various cell lines. Expression of
CDCREL1
was detected in 11 (85%) of 13 AML cell lines and 3 (21%) of 14 acute lymphoblastic leukemia (ALL) cell lines, but none of 11 EB virus transformed B-cell lines by RT-PCR. The expression rate of
CDCREL1
was significantly higher in AML cell lines than in ALL cell lines (P = 0.0035). Platelet glycoprotein 1B beta (GP1B beta), which is located downstream of
CDCREL1
and is cotranscribed with
CDCREL1
due to a nonconsensus polyadenylation sequence, was expressed in all these cell lines. The higher expression rate of
CDCREL1
in AML cell lines than in ALL cell lines suggests that this gene may play some role in myeloid leukemogenesis.
...
PMID:The CDCREL1 gene fused to MLL in de novo acute myeloid leukemia with t(11;22)(q23;q11.2) and its frequent expression in myeloid leukemia cell lines. 1117 Feb 79
