Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.7.6 (
RNA polymerase
)
34,946
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The poliovirus
RNA polymerase
complex has been analyzed by immunoautoradiography using antibody probes derived from purified replicase (P3) region viral polypeptides. Antibody preparations made against the polio
RNA polymerase
, P3-4b, detected a previously unreported cellular protein that copurifies with the
RNA polymerase
. An IgG fraction purified from rabbit antiserum to polypeptide P3-2, a precursor of the
RNA polymerase
, specifically inhibits poliovirus RNA synthesis in vitro. We have also immunoprecipitated a 60,000-dalton protein (P3-4a) with antiserum to protein P3-4b and have determined the precise genomic map position of this protein by automated Edman degradation.
Protein P3
-4a originates by cleavage of the
RNA polymerase
precursor at a glutamine-glycine amino acid pair not previously reported to be a viral cleavage site.
...
PMID:Poliovirus RNA synthesis in vitro: structural elements and antibody inhibition. 630 5
