Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.7.6 (
RNA polymerase
)
34,946
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
More than 200 plants have been suffering from Verticillium wilt caused by
Verticillium dahliae
(
V. dahliae
) across the world. The target of rapamycin (TOR) is a lethal gene and controls cell growth and development in various eukaryotes, but little is known about TOR signaling in
V. dahliae
. Here, we found that
V. dahliae
strain is hypersensitive to rapamycin in the presence of
rapamycin binding protein
VdFKBP12 while the deletion mutant aaa
vdfkbp12
is insensitive to rapamycin. Heterologous expressing
VdFKBP12
in
Arabidopsis
conferred rapamycin sensitivity, indicating that
VdFKBP12
can bridge the interaction between rapamycin and TOR across species. The key across species of TOR complex 1 (TORC1) and TORC2 have been identified in
V. dahliae
, suggesting that TOR signaling pathway is evolutionarily conserved in eukaryotic species. Furthermore, the RNA-seq analysis showed that ribosomal biogenesis,
RNA polymerase II
transcription factors and many metabolic processes were significantly suppressed in rapamycin treated cells of
V. dahliae
. Importantly, transcript levels of genes associated with cell wall degrading enzymes (CWEDs) were dramatically down-regulated in TOR-inhibited cells. Further infection assay showed that the pathogenicity of
V. dahliae
and occurrence of Verticillium wilt can be blocked in the presence of rapamycin. These observations suggested that VdTOR is a key target of
V. dahliae
for controlling and preventing Verticillium wilt in plants.
...
PMID:Functional Characterization of Target of Rapamycin Signaling in
Verticillium dahliae
. 3091 4
