Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.7.6 (
RNA polymerase
)
34,946
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Transcription of protein-coding genes in Leishmania major and other trypanosomatids differs from that in most eukaryotes and bioinformatic analyses have failed to identify several components of the
RNA polymerase
(RNAP) complexes. To increase our knowledge about this basic cellular process, we used tandem affinity purification (TAP) to identify subunits of RNAP II and III. Mass spectrometric analysis of the complexes co-purified with TAP-tagged LmRPB2 (encoded by LmjF31.0160) identified seven RNAP II subunits: RPB1, RPB2, RPB3, RPB5, RPB7, RPB10 and RPB11. With the exception of RPB10 and RPB11, and the addition of RPB8, these were also identified using TAP-tagged constructs of one (encoded by LmjF34.0890) of the two LmRPB6 orthologues. The latter experiments also identified the RNAP III subunits RPC1 (C160), RPC2 (C128), RPC3 (C82),
RPC4
(C53), RPC5 (C37), RPC6 (C34), RPC9 (C17), RPAC1 (AC40) and RPAC2 (AC19). Significantly, the complexes precipitated by TAP-tagged LmRPB6 did not contain any RNAP I-specific subunits, suggesting that, unlike in other eukaryotes, LmRPB6 is not shared by all three polymerases but is restricted to RNAP II and III, while the LmRPB6z (encoded by LmjF25.0140) isoform is limited to RNAP I. Similarly, we identified peptides from only one (encoded by LmjF18.0780) of the two RPB5 orthologues and one (LmjF13.1120) of the two RPB10 orthologues, suggesting that LmRPB5z (LmjF18.0790) and LmRPB10z (LmjF13.1120) are also restricted to RNAP I. In addition to these RNAP subunits, we also identified a number of other proteins that co-purified with the RNAP II and III complexes, including a potential transcription factor, several histones, an ATPase involved in chromosome segregation, an endonuclease, four helicases, RNA splicing factor PTSR-1, at least two RNA binding proteins and several proteins of unknown function.
...
PMID:Characterization of the RNA polymerase II and III complexes in Leishmania major. 1727 24
Reproductive barriers are commonly observed in both animals and plants, in which they maintain species integrity and contribute to speciation. This report shows that a combination of loss-of-function alleles at two duplicated loci,
DUPLICATED GAMETOPHYTIC STERILITY 1
(
DGS1
) on chromosome 4 and
DGS2
on chromosome 7, causes pollen sterility in hybrid progeny derived from an interspecific cross between cultivated rice,
Oryza sativa
, and an Asian annual wild rice,
O. nivara
Male gametes carrying the
DGS1
allele from
O. nivara
(
DGS1-nivara
s
) and the
DGS2
allele from
O. sativa
(
DGS2-T65
s
) were sterile, but female gametes carrying the same genotype were fertile. We isolated the causal gene, which encodes a protein homologous to
DNA-dependent RNA polymerase
(RNAP) III subunit C4 (
RPC4
).
RPC4
facilitates the transcription of 5S rRNAs and tRNAs. The loss-of-function alleles at
DGS1-nivara
s
and
DGS2-T65
s
were caused by weak or nonexpression of
RPC4
and an absence of
RPC4
, respectively. Phylogenetic analysis demonstrated that gene duplication of
RPC4
at
DGS1
and
DGS2
was a recent event that occurred after divergence of the ancestral population of
Oryza
from other Poaceae or during diversification of AA-genome species.
...
PMID:Duplication and Loss of Function of Genes Encoding RNA Polymerase III Subunit C4 Causes Hybrid Incompatibility in Rice. 2859 58
