Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.7.6 (
RNA polymerase
)
34,946
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The human
DHRS4
gene cluster consists of
DHRS4
and two immediately downstream homologous genes, DHRS4L2 and DHRS4L1, generated by evolutionarily gene-duplication events. We previously demonstrated that a head-to-head natural antisense transcript (NAT) of
DHRS4
, denoted
DHRS4
-AS1, regulates all three genes of the
DHRS4
gene cluster. However, it is puzzling that DHRS4L2 and DHRS4L1 did not evolve their own specific NATs to regulate themselves, as it seems both have retained sequences highly homologous to
DHRS4
-AS1. In a search of the
DHRS4
-AS1 region for nearby enhancers, we identified an enhancer located 13.8 kb downstream of the
DHRS4
-AS1 transcriptional start site. We further showed, by using a chromosome conformation capture (3C) assay, that this enhancer is capable of physically interacting with the
DHRS4
-AS1 promoter through chromosomal looping. The enhancer produced an eRNA, termed AS1eRNA, that enhanced
DHRS4
-AS1 transcription by mediating the spatial interactions of the enhancer and
DHRS4
-AS1 promoter in cooperation with
RNA polymerase II
and p300/CBP. Moreover, the distributions of activating acetyl-H3 and H3K4me3 modifications were found to be greater at the
DHRS4
-AS1 promoter than at the homologous duplicated regions. We propose that AS1eRNA-driven DNA looping and activating histone modifications promote the expression of
DHRS4
-AS1 to economically control the
DHRS4
gene cluster.
...
PMID:Enhancer RNA-driven looping enhances the transcription of the long noncoding RNA DHRS4-AS1, a controller of the DHRS4 gene cluster. 2686 44
