Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.7.6 (
RNA polymerase
)
34,946
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Human aminoacyl-tRNA synthetases (ARSs) are normally located in cytoplasm and are involved in protein synthesis. In the present work, we found that human
methionyl-tRNA synthetase
(
MRS
) was translocated to nucleolus in proliferative cells, but disappeared in quiescent cells. The nucleolar localization of
MRS
was triggered by various growth factors such as insulin, PDGF, and EGF. The presence of
MRS
in nucleoli depended on the integrity of RNA and the activity of
RNA polymerase I
in the nucleolus. The ribosomal RNA synthesis was specifically decreased by the treatment of anti-
MRS
antibody as determined by nuclear run-on assay and immunostaining with anti-Br antibody after incorporating Br-UTP into nascent RNA. Thus, human
MRS
plays a role in the biogenesis of rRNA in nucleoli, while it is catalytically involved in protein synthesis in cytoplasm.
...
PMID:Nucleolar localization of human methionyl-tRNA synthetase and its role in ribosomal RNA synthesis. 1079 71
