Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:2.7.7.6 (RNA polymerase)
 34,946 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Total mRNA of Candida strains( isolated form whole vaginal swabs) was investigated and the in vivo expression of C. albicans secreted aspartyl proteinase (SAP4), aglutinin-like sequence (ALS1) and hyphal wall protein (HWP1) genes was determined. A spectrum of gene expression profiles of strain isolated from vulvovaginal candidiasis(VVC) cases consisting of 10 pregnant, 4 postmenopausal, and 15 reproductive age (12 primary and 3 recurrent) 9 women with different estrogen level. Expression of SAP4, ALS1 and HWP1 genes was evaluated by reverse-transcriptase polymerase chain reaction using specific primer sets. The expression of ALS1, HWP1, and SAP4 was detected as 69, 62, and 38 %, respectively, in all cases. In pregnant, postmenopausal, and reproductive age women with VVC, the expression of ALS1 was observed as 70, 75, 67%, and HWP 60, 25, 73% respectively . Expression of SAP4 was found in pregnant, postmenopausal, and reproductive age women as 40, 50, and 33% respectively. Expression of teh adhesion genes in VVC does not correlate with estrogen level of patients.
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PMID:Expression of ALS1, HWP1 and SAP4 genes in Candida albicans strains isolated from women with vaginitis. 1883 69

The Mediator complex is an essential co-regulator of RNA polymerase II that is conserved throughout eukaryotes. Here we present the first study of Mediator in the pathogenic fungus Candida albicans. We focused on the Middle domain subunit Med31, the Head domain subunit Med20, and Srb9/Med13 from the Kinase domain. The C. albicans Mediator shares some roles with model yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe, such as functions in the response to certain stresses and the role of Med31 in the expression of genes regulated by the activator Ace2. The C. albicans Mediator also has additional roles in the transcription of genes associated with virulence, for example genes related to morphogenesis and gene families enriched in pathogens, such as the ALS adhesins. Consistently, Med31, Med20, and Srb9/Med13 contribute to key virulence attributes of C. albicans, filamentation, and biofilm formation; and ALS1 is a biologically relevant target of Med31 for development of biofilms. Furthermore, Med31 affects virulence of C. albicans in the worm infection model. We present evidence that the roles of Med31 and Srb9/Med13 in the expression of the genes encoding cell wall adhesins are different between S. cerevisiae and C. albicans: they are repressors of the FLO genes in S. cerevisiae and are activators of the ALS genes in C. albicans. This suggests that Mediator subunits regulate adhesion in a distinct manner between these two distantly related fungal species.
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PMID:The functions of Mediator in Candida albicans support a role in shaping species-specific gene expression. 2249 66