Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.7.6 (
RNA polymerase
)
34,946
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Spt3 is a Saccharomyces cerevisiae transcription factor that is required in vivo for the transcription of a number of
RNA polymerase II
-transcribed genes. We report the cloning of the gene encoding the human homologue of Spt3,
SUPT3H
, and its initial functional analysis. The human and yeast Spt3 homologues share an overall identity of 30% that defines three conserved regions, suggesting possible functional domains. To determine whether
SUPT3H
is a true functional Spt3 homologue, we tested for complementation of an spt3Delta mutation in yeast. While expression of the full-length
SUPT3H
is unable to complement an spt3Delta mutation, expression of a human-yeast chimeric gene that contains 42% human sequences can partially complement an spt3Delta mutation. These data suggest that the transcriptional control carried out by Spt3 has been conserved from yeast to human.
...
PMID:Characterization of a human homologue of the Saccharomyces cerevisiae transcription factor spt3 (SUPT3H). 978 80
