Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.7.49 (
reverse transcriptase
)
31,746
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The identification and study of genes expressed in hematopoietic stem/progenitor cells should further our understanding of hematopoiesis. Transcription factors in particular are likely to play important roles in maintaining the set of genes that define the stem/progenitor cell. We report here the identification of a putative KRAB-zinc finger gene (
SZF1
) from a cDNA library prepared from human bone marrow CD34+ cells. Characterization of
SZF1
implicates its role in hematopoiesis. The predicted protein contains a highly conserved KRAB domain at the NH2 terminus and four zinc fingers of the C2H2 type at the COOH terminus. Two alternatively spliced products of
SZF1
were isolated, which predict proteins of 421 (SZF1-1) and 361 (SZF1-2) amino acids, differing from each other only at the carboxy terminus. The two transcripts of
SZF1
have different expression patterns.
SZF1
-2 is ubiquitously expressed, as indicated by Northern blot, RNase protection, and
reverse transcriptase
polymerase chain reaction.
SZF1
-1 expression, in contrast, was detected only in CD34+ cells. We recently isolated the promoter region for the stem/progenitor cell expressed FLT3/FLK-2/STK-1 gene and used this region to generate a reporter construct to test the effect of
SZF1
expression. Cotransfection of the reporter construct with
SZF1
constructs showed that
SZF1
-2 repressed transcription three- to fourfold, whereas
SZF1
-1 showed a lower level of repression. The expression pattern of
SZF1
transcripts and the transcriptional repression of a CD34+-specific promoter demonstrate a possible role for
SZF1
in hematopoietic stem/progenitor cell differentiation.
...
PMID:SZF1: a novel KRAB-zinc finger gene expressed in CD34+ stem/progenitor cells. 1002 71
