Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.7.49 (
reverse transcriptase
)
31,746
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A new DNA polymerase (peak A) has been identified in the high-speed pellet fraction of two subclones of nonvirus producing Balb/3T3 cells. The activity is associated with a molecule of approximately 70,000 molecular weight and chromatographs in two systems like the mouse type-C viral
reverse transcriptase
and a similar enzyme from the high-speed pellet fraction of a virus producing Balb/3T3 subclone, S(2)Cl(3). Comparable quantities of peak A are present in both nonvirus infected (A31) and mouse sarcoma virus transformed nonproducer (KA31) subclones. Virus producing cells contain 10-20 times more peak A polymerase activity. A31 and KA31 peak A are comparably inhibited by anti-mouse type C virus
reverse transcriptase
IgG but to a lesser degree than S(2)Cl(3) peak A or authentic viral
reverse transcriptase
. They can also be differentiated from the latter two enzymes by template preference studies. KA31 peak A can be distinguished from three other KA31 DNA polymerases (R-DNA polymerase and
DNA polymerase N
and C), and thus appears to be a new species of cellular DNA polymerase.
...
PMID:Characterization of a new murine cellular DNA polymerase. 412 4
