Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:2.7.7.49 (reverse transcriptase)
 31,746 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Dopamine acts, under appropriate conditions, as a selective neurotoxin. This toxicity is attributed to the autoxidation of the neurotransmitter into a reactive quinone that covalently modifies cellular macromolecules (i.e. proteins and nucleic acids). The oxidation of the catecholamine to a quinone is greatly accelerated by the enzyme tyrosinase. There is controversy, however, as to whether or not tyrosinase is expressed in human brain. In the present study, RT-PCR was utilized to demonstrate the presence of tyrosinase mRNA in post-mortem human brain tissues. Using gene-specific amplification primers, specific tyrosinase amplicons were detected following analysis of RNA from substantia nigra of four individuals. Analysis of cerebellar RNA from the same individuals produced no amplification products. Control reactions performed in the absence of reverse transcriptase failed to generate PCR products for any tissue tested. Three amplicons were subjected to direct DNA sequencing and all proved to be identical with tyrosinase sequences, thus obviating the possibility of amplification of a related gene. It is clear, therefore, that the tyrosinase gene is expressed in the human substantia nigra, lending support to previous studies describing tyrosinase-like activity and immunoreactive protein in the brain. This enzyme could be central to dopamine neurotoxicity as well as contribute to the neurodegeneration associated with Parkinson's disease.
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PMID:Tyrosinase mRNA is expressed in human substantia nigra. 910 85

In ectothermic vertebrates, visceral organs harbor melanin-containing cells. Their ability as pigment producers is nevertheless disputed. To address expression of the key genes for melanogenesis in Atlantic salmon (Salmo salar), a tyrosinase-positive leukocyte cell line (SHK-1) and skin were used to obtain full-length tyrosinase (Tyr), tyrosinase-like protein-1 (Tyrp1), and dopachrome tautomerase (Dct) mRNA transcripts. In the SHK-1 cells, two different Tyrp1 transcripts were identified, one lacking exon 1. However, only the full-length version of Tyrp1 was identified in the skin. Sequencing of Tyrp1 genomic region revealed that the two Tyrp1 transcripts might originate from two different loci, possibly a result of pseudo-tetraploidity of the Atlantic salmon genome. Expression of Tyr, Tyrp1 and Dct was investigated by quantitative real-time reverse transcriptase polymerase cain reaction showing highest expression in the SHK-1 cell line and skin, intermediate in pronephros, and negligible or absent in liver and muscle. Histological approaches were used to demonstrate melanin and revealed presence of melanized cells in skin, kidney and liver, and absence of such cells in muscle. In addition to verify melanin synthesis abilities of visceral-located cells, our results indicate loci-specific transcription differences between populations of melanin-producing cells in Atlantic salmon.
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PMID:Isolation of the Atlantic salmon tyrosinase gene family reveals heterogenous transcripts in a leukocyte cell line. 1682 51