Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:2.7.7.49 (reverse transcriptase)
 31,746 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

SPLUNC1, originally named PLUNC for palate, lung and nasal epithelium clone, is a small protein which is secreted from the epithelial cells of the nasal cavity and the upper respiratory tract in humans, mice, rats and cows. SPLUNC1 is structurally homologous to the two key mediators of host defense against Gram-negative bacteria, lipopolysaccharide binding protein (LBP) and bactericidal permeability increasing protein (BPI). SPLUNC1 is therefore believed to play a role in the innate immune system. This work reports the cloning and analysis of the porcine (Sus scrofa) homologue of SPLUNC1. The SPLUNC1 cDNA was amplified by reverse transcriptase polymerase chain reaction (RT-PCR) using oligonucleotide primers derived from in silico sequences. The porcine cDNA codes for a protein of 249 amino acids which shows a high similarity to bovine (74%) and to human (69%) SPLUNC1. The predicted S. scrofa SPLUNC1, SsSPLUNC1, polypeptide contains a putative signal peptide of 19 residues. A similar signal sequence is also found in all other members of the PLUNC family. Expression analysis by RT-PCR demonstrated a very high expression level of the porcine SPLUNC1 homologue in trachea and lung tissue only. This airway-specific expression might be of particular interest in the study of airborne diseases in pig.
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PMID:Porcine SPLUNC1: molecular cloning, characterization and expression analysis. 1577 15

Identification of molecular markers often leads to important clinical applications such as early diagnosis, prognosis, and drug targeting. Lung cancer, the leading cause of cancer-related deaths, still lacks reliable molecular markers. We have combined the bioinformatics analysis of the public gene expression data and clinical validation to identify biomarker genes for non-small-cell lung cancer. The serial analysis of gene expression and the expressed sequence tag data were meta-analyzed to produce a list of the differentially expressed genes in lung cancer. Through careful inspection of the predicted genes, we selected 20 genes for experimental validation using semiquantitative reverse transcriptase-PCR. The microdissected clinical specimens used in the study consisted of three groups: lung tissues from benign diseases and the paired (cancer and pathologic normal) tissues from non-small-cell lung cancer patients. After extensive statistical analyses, seven genes (CBLC, CYP24A1, ALDH3A1, AKR1B10, S100P, PLUNC, and LOC147166) were identified as potential diagnostic markers. Quantitative real-time PCR was carried out to additionally assess the value of the seven identified genes leading to the confirmation of at least two genes (CBLC and CYP24A1) as highly probable novel biomarkers. The gene properties of the identified markers, especially their relationship to lung cancer and cell signaling pathway regulation, further suggest their potential value as drug targets as well.
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PMID:Clinical validity of the lung cancer biomarkers identified by bioinformatics analysis of public expression data. 1767 Dec 13

Idiopathic pulmonary fibrosis (IPF) in dogs is a rare disease of unknown aetiology, seen in terrier breeds, particularly the West Highland white terrier (WHWT). The aim of this study was to determine pulmonary gene expression in canine IPF in order to gain insights into the pathogenesis of the disease and to identify possible biomarkers. Microarray analyses were conducted to determine gene expression profiles in the lungs of dogs with IPF and control dogs of various breeds. More than 700 genes were identified as having greater than two-fold difference in expression between the two groups. The significant biological functions associated with these genes were related to cellular growth and proliferation, developmental processes, cellular movement, cell to cell signalling and interaction, and antigen presentation. Altered levels of expression were confirmed by quantitative reverse transcriptase PCR for genes encoding chemokine (C-C) ligand (CCL) 2 (+4.9 times), CCL7 (+6.8 times), interleukin 8 (+4.32 times), chemokine (C-X-C) ligand 14 (+3.4 times), fibroblast activation protein (+4.7 times) and the palate, lung and nasal associated protein (PLUNC, -25 times). Serum CCL2 concentrations were significantly higher in WHWTs with IPF (mean 628.1 pg/mL, interquartile range 460.3-652.7 pg/mL) than unaffected WHWTs (mean 344.0 pg/mL, interquartile range 254.5-415.5 pg/mL; P=0.001). The results support CCL2 as a candidate biomarker for IPF in dogs.
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PMID:Analysis of gene expression in canine idiopathic pulmonary fibrosis. 2412 Apr 50