Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.7.49 (
reverse transcriptase
)
31,746
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
We have set up an experimental system to map the primary transcription unit of the human
alpha 2 globin
gene. The duplicated human alpha globin genes (alpha 2-alpha 1) were linked to the alpha globin locus Positive Regulatory Element (PRE) and stably transfected into murine erythroleukaemia cells. We then developed a quantitative
reverse transcriptase
, polymerase chain reaction assay to map alpha 2 primary transcripts using primer pairs derived from different parts of the
alpha 2 globin
gene and its 3' flanking region. This approach has revealed the presence of steady state nuclear RNA past the poly(A) site of the
alpha 2 globin
gene at approximately 40% of the level of unspliced intron transcript. Furthermore, these 3' flanking transcripts diminish 500 bp into the 3' flanking region, identifying this part of the
alpha 2 globin
gene as the principal region of termination of transcription.
...
PMID:The primary transcription unit of the human alpha 2 globin gene defined by quantitative RT/PCR. 137 68
