Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:2.7.7.49 (reverse transcriptase)
 31,746 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A Trypanosoma brucei repeated DNA element (TRS-1, for trypanosome repeated sequence), which seems transposable, may encode a 1651 amino acid polypeptide showing homology with reverse transcriptase. This polypeptide would also carry a DNA-binding domain, as suggested by the presence of five DNA-binding "fingers" homologous to those of the transcription factor TFIIIA of Xenopus laevis and retroviral DNA-binding proteins.
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PMID:DNA-binding fingers encoded by a trypanosome retroposon. 244 94

The genome of Trypanosoma brucei contains up to 400 copies of a conserved sequence (TRS, trypanosome repeated sequence). The majority of TRS copies (TRS1) are 5.2 X 10(3) base-pairs (kb) and are flanked by different separate halves of the previously described transposable element RIME (ribosomal mobile element), although a variant copy (TRS2) contains only the central 1.45 kb portion and lacks RIME. TRS1 elements can probably undergo transposition, since they are dispersed in all chromosome size classes and are bordered by direct repeats of about four base-pairs. Some TRS1 elements may contain an open reading frame over almost their entire length (1651 codons), encoding a protein showing homology with reverse transcriptase. TRS probes detect poly(A)+ transcripts of 5 to 9 kb, generated by a polymerase moderately sensitive to alpha-amanitin. Transcription is developmentally regulated. Both TRS and RIME sense transcripts are preferentially synthesized compared to anti-sense transcripts, and are much more abundant in bloodstream forms than in cultured procyclics.
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PMID:Trypanosoma brucei repeated element with unusual structural and transcriptional properties. 365 36

The genome of Trypanosoma brucei contains many copies of TRS/ingi, a retroposon like element of about 5 kb length. One vestigial member of this family, TUBIS, is found in the tubulin gene cluster. The element has sequence homologies to reverse transcriptase. The similarity to TRS/ingi is lost after 4 kb, resulting in a 3' truncated element. The sequence following downstream shows similarities to 5' flanking regions of VSG genes.
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PMID:TUBIS, a fossilized retroposon in the tubulin gene cluster of Trypanosoma brucei. 819 71