Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.7.49 (
reverse transcriptase
)
31,746
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Taurine is thought to be an osmolyte in the kidney medulla. We have investigated the gene expression of the taurine transporter (TauT) and the enzymes of taurine biosynthesis,
cysteine dioxygenase
(
CDO
) and cysteine sulfinate decarboxylase (CSD). We achieved this by measuring their mRNA levels using
reverse transcriptase
polymerase chain reaction (RT-PCR) in five kidney regions of rats in various hydration states; namely, normal hydration, after 2 days of antidiuresis following chronic diuresis and finally after acute salt loading. The mRNA levels of the well-established tonicity-sensitive genes coding for the aldose reductase (AR), the sodium myo-inositol transporter (SMIT) and the betaine transporter (BGT1) were also determined for the sake of comparison. In normally hydrated rats, TauT-,
CDO
-, and CSD-mRNA were enriched in the outer stripe of the outer medulla (OS). Following antidiuresis, the mRNA levels of TauT,
CDO
, CSD, SMIT, BGT1 and AR were all similarly increased in the papilla when compared with levels in rats submitted to a chronic diuresis. After acute salt loading, the mRNA level of TauT, like that of SMIT and BGT1, was overexpressed in OS whereas the mRNA levels of
CDO
and CSD remained unchanged. Like SMIT, BGT1 and AR genes, TauT,
CDO
and CSD genes appear to be tonicity-sensitive genes which can be activated in vivo by hypertonicity in the rat kidney. However, tonicity-induced activation of the TauT gene is more sensitive than that of
CDO
and CSD genes.
...
PMID:Gene expression of the taurine transporter and taurine biosynthetic enzymes in rat kidney after antidiuresis and salt loading. 1137 73
