Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.7.49 (
reverse transcriptase
)
31,746
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The aim of this study was to investigate the potential role of
PHRF1
in lung tumorigenesis. Western blot analysis was used to detect the expression of proteins. Quantitative
reverse transcriptase
polymerase chain reaction, immunohistochemistry, soft agar assay and tumor formation assay in nude mice were applied. Cell cycle distribution was analyzed by flow cytometry. The lower level of
PHRF1
mRNA was observed in human lung cancer tissues than that in paracancerous tissues. The decreased expression of
PHRF1 protein
was observed in H1299 and H1650 cell lines than that in 16HBE and BEAS-2B cell lines. The decreased expression of
PHRF1 protein
was observed in malignant 16HBE cells compared to control cells. The reduced expression of
PHRF1 protein
was observed in mice lung tissues treated with BaP than that in control group. Overexpression of
PHRF1
inhibited H1299 cell proliferation, colony formation in vitro and growth of tumor xenograft in vivo, and arrested cell cycle in G1 phase. The decreased expression of TGIF and c-Myc proteins and the increased expression of p21 protein were observed in H1299-
PHRF1
cells compared with H1299-pvoid cells. In conclusion, our findings suggest that overexpression of
PHRF1
attenuated the proliferation and tumorigenicity of non-small cell lung cancer cell line of H1299.
...
PMID:Overexpression of PHRF1 attenuates the proliferation and tumorigenicity of non-small cell lung cancer cells. 2760 40
Complex cytoplasmic nucleotide-sensing mechanisms can recognize foreign DNA based on a lack of methylation and initiate an immune response to clear the infection. Zebrafish embryos with global DNA hypomethylation caused by mutations in the
u
biquitin-like with
PHD and ring finger domains 1
(
uhrf1
) or
DNA methyltransferase 1
(
dnmt1
) genes exhibit a robust interferon induction characteristic of the first line of defense against viral infection. We found that this interferon induction occurred in non-immune cells and examined whether intracellular viral sensing pathways in these cells were the trigger. RNA-seq analysis of
uhrf1
and
dnmt1
mutants revealed widespread induction of Class I retrotransposons and activation of cytoplasmic DNA viral sensors. Attenuating Sting, phosphorylated Tbk1 and, importantly, blocking
reverse transcriptase
activity suppressed the expression of interferon genes in
uhrf1
mutants. Thus, activation of transposons in cells with global DNA hypomethylation mimics a viral infection by activating cytoplasmic DNA sensors. This suggests that antiviral pathways serve as surveillance of cells that have derepressed intragenomic parasites due to DNA hypomethylation.
...
PMID:Loss of DNA methylation in zebrafish embryos activates retrotransposons to trigger antiviral signaling. 2869 26
