Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:2.7.7.49 (reverse transcriptase)
 31,746 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We identified an alternative mRNA encoding a novel cadherin-7 isoform by reverse transcriptase-PCR of RNA from day 12 chicken embryos. The alternative mRNA contains 49 bases of insertion in the premembrane region, leading to the substitution of 14 amino acids and the introduction of a premature stop codon. Identification of a 49-bp insertion sequence in the genomic DNA corresponding to the intron of the cadherin-7 gene suggests that alternative splicing is the cause of the alternative mRNA. Transient expression of the variant form in COS-7 or 293 cells produced a soluble protein. Aggregation assays and immunoprecipitation showed that the variant protein interacts with full-length cadherin-7 in vitro and in vivo and inhibits full-length cadherin-7-mediated cell adhesion. Immunohistochemistry revealed that the variant form was strongly expressed in dermomyotomes rather than in migrating neural crest cells, in contrast to the full-length cadherin-7, suggesting differential regulation of splicing and possible roles of variant cadherin-7 in the development of dermomyotomes and other tissues.
 ...
PMID:Identification and characterization of a soluble cadherin-7 isoform produced by alternative splicing. 1236 38

We cloned chicken cadherin-19 that demonstrates high similarity to human and rat cadherin-19. Chicken cadherin-19 is a type II classic cadherin that is located on the long arm of chicken chromosome 2 and is composed of 13 exons and 12 introns. The expression profile of cadherin-19 was analyzed by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and in situ hybridization during chicken embryonic development. Its expression starts at E2.5, then gradually increases to reach a peak at E20. In contrast to previous results obtained in rat, chicken cadherin-19 is expressed both in Schwann cells and oligodendrocytes, also at late stages of development. We found no other cell type positive for cadherin-19 in the chicken embryo throughout development, suggesting that cadherin-19 is selectively expressed by myelin-forming cells and might play a role in myelin formation. The sequence of cadherin-19 shares high similarity with that of cadherin-7 and cadherin-20, and the three genes form a cluster on chromosome 2. Their expression patterns, however, are rather distinct although partial overlap is observed. For example, cadherin-19 and cadherin-7 are co-expressed by Schwann cells but not by oligodendrocytes. Moreover, a subset of interneurons express cadherin-7 but not cadherin-19 or cadherin-20. Despite their close genetic relation, the three cadherins have acquired functions in rather different cell types during nervous system development.
 ...
PMID:Cadherin-19 expression is restricted to myelin-forming cells in the chicken embryo. 1985 Jan 11