Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.7.49 (
reverse transcriptase
)
31,746
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
In order to investigate how glucocorticoids affect the expression of
secretory leukocyte peptidase inhibitor
(
SLPI
), which is overexpressed in a variety of cancers, clobetasol was added to cell culture medium of U937 cells and the
SLPI
mRNA levels were examined. The in vitro effect of the treatment on
SLPI
expression was detected by
reverse transcriptase
-polymerase chain reaction. Clobetasol treatment of U937 cells induced an up- and down-regulation of
SLPI
expression in a dose-dependent manner. Western blotting confirmed the down-regulation of
SLPI
protein expression. We hypothesized a loop formation in the
SLPI
genome domain, in which the glucocorticoid receptor regulates bi-directional transcriptional activity.
...
PMID:Clobetasol down-regulates SLPI expression in U937 monocytoid cells. 2207 18
