Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:2.7.7.49 (reverse transcriptase)
 31,746 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

During the infantile period of the female rat (8-21 postnatal days [PND] of age), there is a dramatic increase in plasma FSH, which is thought to be important in initiating ovarian activity and, perhaps, the onset of puberty. To begin to understand the regulation of this FSH surge, we determined the ontogenetic development of LHbeta, FSHbeta, and GnRH receptor (GnRH-R) mRNA levels in the pituitary gland throughout the infantile period of the female rat. Steady-state mRNA levels were determined by an external standard quantitative reverse transcriptase polymerase chain reaction assay. FSHbeta and GnRH-R mRNA levels increased to peak on PND 12 (p < 0.03). LHbeta mRNA levels remained relatively constant until rising on PND 18. A GnRH antagonist (10-100 microg/animal) was administered daily from PND 8-11 or PND 11-13, and animals were killed on PND 12 or PND 14, respectively. FSHbeta, LHbeta, and GnRH-R mRNAs were not affected by GnRH antagonist treatment. Plasma FSH was selectively reduced in the first group, whereas both plasma LH and FSH were suppressed in the second group. These data indicate that gene expression of LHbeta, FSHbeta, and GnRH-R are differentially regulated in the infantile female rat pituitary. GnRH is involved in regulating the secretion of FSH and LH during the infantile period but not in regulating FSHbeta, LHbeta, or GnRH-R mRNA gene expression.
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PMID:Ontogeny of gene expression in the gonadotroph of the developing female rat. 911 62

Effects of age on uterine histoarchitecture, cell proliferation, and hormone receptor expression were determined for neonatal ewe lambs from birth (Postnatal Day [PND] 0) to PND 56. Uteri were histologically evaluated and proliferating cell nuclear antigen (PCNA), estrogen receptor alpha (ER-alpha), progesterone receptor (PR), and prolactin receptor (PRL-R) expression were characterized by in situ hybridization (ISH), immunohistochemistry, or both. The most striking feature of neonatal uterine development was the genesis and development of glands in the intercaruncular areas of endometrium. After birth, endometrial glandular epithelium (GE) budded and differentiated into the underlying stroma from the luminal epithelium (LE) between PNDs 1 and 7. Between PNDs 14 and 56, extensive coiling and branching morphogenesis of nascent endometrial glands occurred. By PND 56, the uterine wall appeared to be histoarchitecturally mature. At birth, nuclear PCNA protein was strongly detected in LE. Between PNDs 7 and 56, high levels of PCNA, ER-alpha, and PR gene expression were detected in both nascent and developing GE. Higher levels of PCNA and ER-alpha expression were detected in GE at the tips of developing glands as well as in the surrounding stroma. Progesterone was below detectable limits in serum. Serum estradiol-17beta levels were high on PND 1, increased from PNDs 14 to 28, and declined from PND 42 to PND 56. Serum PRL levels increased from PNDs 1 to 14 and declined thereafter. Using ISH and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis, expression of mRNAs for short and long forms of the ovine PRL-R were first detected in nascent GE on PND 7 and increased between PNDs 7 and 56 in proliferating and differentiating GE. These results indicate that 1) uterine gland genesis is initiated between PNDs 1 and 7 after birth and is essentially completed by PND 56; 2) neonatal uterine morphogenesis involves temporal and spatial alterations in cell proliferation and ER-alpha, PR, and PRL-R gene expression; 3) PRL-R expression is a unique marker of GE differentiation and proliferation; and 4) serum estradiol-17beta and PRL levels increase during the onset of GE tubular branching morphogenesis. Results support the hypothesis that neonatal ovine uterine development involves epithelial PRL-R and ER-alpha activation to stimulate and maintain endometrial gland genesis and branching morphogenesis.
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PMID:Neonatal ovine uterine development involves alterations in expression of receptors for estrogen, progesterone, and prolactin. 1099 45

Treatment of pregnant seropositive women and their neonates with the nucleoside analogs (reverse transcriptase inhibitors) zidovudine (AZT), lamivudine (3TC) and their combination has become a standard of care in industrialized countries to prevent transmission of the HIV-1 virus. Animal studies indicated limited but significant behavioral changes in AZT or 3TC-prenatally exposed offspring, whereas data on the potential neurobehavioral outcomes of AZT+3TC combination are still lacking. The aim of the present study was to assess in mice prenatally exposed to AZT+3TC the functional state of cholinergic muscarinic neuroregulation at adulthood. Pregnant CD-1 mice received per orem twice daily AZT+3TC (160 and 500 mg/kg, respectively) or vehicle solution (NaCl 0.9%) from gestational day (GD) 10 to delivery (GD 19). Locomotor activity, exploratory behavior and responsiveness to the muscarinic cholinergic blocker scopolamine (2 mg/kg) were analyzed at adulthood (PND 70) in offspring of both sexes in an open field test. Results indicated that prenatal AZT+3TC exposure does not influence responsiveness to the muscarinic cholinergic antagonist as measured by analysis of the drug's effects on locomotor and exploratory activity and different behavioral items. However, AZT+3TC-treated mice displayed higher frequency of rearing, and lower frequency and duration of self-grooming behavior, consistent with an effect on dopaminergic neurotransmission. However, this would need confirmatory experiments.
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PMID:Effects of prenatal AZT+3TC treatment on open field behavior and responsiveness to scopolamine in adult mice. 1116 81