Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.7.48 (
transcriptase
)
9,479
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The effect of methylglyoxal bis(guanylhydrazone) (
MGBG
), a structural analog of polyamines, on protein synthesis has been studied in the presence and absence of spermidine. The spermidine stimulation of polyphenylalanine- and MS2 RNA-directed
RNA replicase
synthesis in an Escherichia coli cell-free system and of globin synthesis in a rabbit reticulocyte cell-free system disappeared with the addition of
MGBG
. The spermidine reduction of misincorporation of leucine during polyphenylalanine synthesis in both E. coli and wheat germ cell-free systems was also disturbed by
MGBG
.
MGBG
noncompetitively interfered with polyamine stimulation of polyphenylalanine and globin synthesis, suggesting that
MGBG
could bind to both RNA and the complex of RNA and polyamine.
MGBG
was preferentially bound to ribosomal RNA among ribosomal RNA, poly(U), and calf thymus DNA, and strongly inhibited the amount of polyamine bound to ribosomal RNA. These results suggest that
MGBG
elimination of polyamine effects on protein synthesis may occur through the disturbance of polyamine binding to ribosomal RNA.
...
PMID:Methylglyoxal bis(guanylhydrazone) elimination of polyamine effects on protein synthesis. 241 7
