Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.7.48 (
transcriptase
)
9,479
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A substantial amount of
cytochrome oxidase subunit III
(
COIII
) mRNA continues to be synthesized de novo in Trypanosoma brucei in the presence of actinomycin D, presumably by a DNA-independent transcription process. We describe the identification of negative-strand
COIII
RNA molecules, characterization of their termini, and the detection of
RNA-dependent RNA polymerase
activity. Three lines of evidence for the existence of negative-strand
COIII
RNA are presented: (i) hybridization with oligonucleotide probes with the same polarity as mRNA after preliminary enrichment for putative negative-strand RNA by affinity purification; (ii) cloning and sequencing of negative-strand complements for the unedited, edited, and partially edited
COIII
RNA; and (iii) exact correspondence of the terminal sequences of the putative negative-strand RNA molecules to the ends of
COIII
RNA. The presence of negative-strand complements of
COIII
RNA is consistent with the notion that a significant amount of mRNA in T. brucei is synthesized by an
RNA-dependent RNA polymerase
with negative-strand RNA as an intermediate template.
...
PMID:Identification of negative-strand complements to cytochrome oxidase subunit III RNA in Trypanosoma brucei. 166 Jan 45
