Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.7.48 (
transcriptase
)
9,479
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
An assay for flavivirus
RNA-dependent RNA polymerase
activity in vitro was established using extracts of Vero cells infected with dengue virus type 2 (DEN-2) or Kunjin virus (KUN). RNA synthesis was initiated on a template of viral replicative form (RF) and RF was converted to the replicative intermediate (RI). The
RNA-dependent RNA polymerase
complex of
DEN
-2 utilised either
DEN
-2 or KUN RF as template, and similarly the KUN polymerase complex utilised either
DEN
-2 or KUN RF template. In addition, antibodies against the nonstructural proteins NS3 and NS5 inhibited the conversion of RF to RI, indicating that NS3 and NS5 are involved in viral RNA replication.
...
PMID:Synthesis of dengue virus RNA in vitro: initiation and the involvement of proteins NS3 and NS5. 841 88
Field male Aedes aegypti (L.) and Aedes albopictus (Skuse) adults caught from fixed monitoring stations weekly for 1 yr were screened for dengue viruses (
DEN
-1,
DEN
-2,
DEN
-3, and
DEN
-4). The assay was carried out using a single-step reverse transcription (or
transcriptase
)-polymerase chain reaction (PCR) (RT-PCR) followed by a semi-nested PCR using an upstream consensus primer and four type-specific primers within the nonstructural protein three gene (NS3) of dengue viruses. The diagnostic fragments for
DEN
-1,
DEN
-2,
DEN
-3, and
DEN
-4 serotypes were of sizes 169, 362, 265, and 426 bp, respectively. Results showed that in Singapore 1.33% and 2.15% of Aedes aegypti and Aedes albopictus adult male mosquitoes, respectively, were positive for dengue viruses. The serotypes detected in male Ae. aegypti was
DEN
-1 (44%), followed by
DEN
-2 (22.2%) and
DEN
-3 (22.2%), and
DEN
-4 (11.1%). For Aedes albopictus males, the serotype was
DEN
-4 (38.9%), followed by
DEN
-2 (33.3%),
DEN
-3 (16.7%), and
DEN
-1 (11.1%).
...
PMID:Detection of dengue viruses in field caught male Aedes aegypti and Aedes albopictus (Diptera: Culicidae) in Singapore by type-specific PCR. 1147 26
