Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:2.7.7.48 (transcriptase)
 9,479 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A novel variant of endothelin B receptor (ETB) has been found in human brain, placenta, lung, and heart by reverse transcriptase polymerase chain reaction. This variant ETB1 has an additional 30 nucleotide sequence with splice sites at both ends. This results in a 10 amino acid increase in the length of the second cytoplasmic domain of ETB. Polymerase chain reaction on genomic DNA indicates that this sequence is part of the 134 bp intron which separates the second and third exons and is contiguous with the third exon of the ETB gene. Southern blot analysis of chromosomal DNA and genomic PCR results indicate that ETB1 arises by alternative RNA splicing of the single copy ETB gene. The insert sequence in ETB1 gene is absent in bovine, rat, and porcine DNA, and is unique to human DNA. Both ETB and ETB1 have been expressed in heterologous systems to examine their ligand binding and functional properties. Reverse transcriptase polymerase chain reaction of RNA from ETB1 expressing cells indicates that the additional sequence is stably expressed.
 ...
PMID:Two distinct human endothelin B receptors generated by alternative splicing from a single gene. 786 30

Endothelin receptors have been characterized in human temporal artery by molecular biological methods and in vitro pharmacology. Reverse transcriptase-polymerase chain reaction was used to detect mRNA encoding ETA and ETB receptors in normal and endothelium-denuded arteries. Vasomotor response experiments with a specific ETA antagonist (FR 139317) suggested the presence of ETA subtypes. Marked ETB-mediated relaxation was obtained with ET-3 when ETA activity was blocked in precontracted arteries. Relaxation was significantly reduced by bosentan, indomethacin, and a nitric oxide synthase inhibitor. It may be speculated that the relaxant activity is mediated through ETB1 receptors.
 ...
PMID:Relaxation of human temporal artery by endothelin ETB receptors. 895 48