Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.7.48 (
transcriptase
)
9,479
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Dehydroepiandrosterone
(
DHEA
) is a steroid hormone produced by the adrenal cortex that serves as an intermediary in sex steroid synthesis.
DHEA
is produced in abundance by humans and most other warm-blooded animals. Based upon previous reports demonstrating the antiviral and immunostimulatory activities of
DHEA
and
DHEA
-sulfate (DHEAS) we sought to determine whether introduction of these compounds would affect replication of feline immunodeficiency virus (FIV) in chronically infected cells. When cell number, cell viability, cellular DNA synthesis, and levels of FIV reverse-
transcriptase
(RT) were measured in cell cultures treated with various dilutions of
DHEA
or DHEAS it was found that the production of FIV RT was inhibited by
DHEA
at levels where cellular viability and DNA synthesis were not affected. At the concentrations tested DHEAS did not inhibit FIV replication or impact on cellular viability or proliferation.
...
PMID:Dehydroepiandrosterone inhibits replication of feline immunodeficiency virus in chronically infected cells. 754 11
Sulphotransferases are a family of enzymes involved in the metabolism and detoxification of many compounds.
Dehydroepiandrosterone
(
DHEA
) sulphotransferase (DHEA-ST), which catalyzes the sulphation of steroids such as
DHEA
, is present in rat liver and adrenals. Sulphated steroids are present in urine, and many other enzymes which catalyze detoxification reactions are found in the kidney. There are not previous reports of DHEA-ST localization in adult kidney. The activity of DHEA-ST was investigated in adult rat kidney by a radio-isotope assay with
DHEA
as the substrate. Western blotting was used to assess protein expression, and the localization of DHEA-ST was investigated by immunohistochemistry. The DHEA-ST activity in rat kidney was found to be approximately four times less than that in rat liver. In female kidney, the activity was 1.46 +/- 0.06 nmol/min/microg, and in male kidney the activity was 1.29 +/- 0.09 nmol/min/microg. Investigation of protein expression gave a single band at 35 kDa which signified the presence of this enzyme in both male and female adult rat kidneys. Localization studies showed positive staining at high intensity in the collecting ducts of the medulla and in the S3 portion of the proximal convoluted tubule in the cortex. The distribution within the proximal tubules was restricted to the brush border. Reverse-
transcriptase
polymerase chain reaction showed DHEA-ST RNA expression in adult rat kidney and liver. The presence of this enzyme and its location in the kidney may suggest that in situ sulphation via DHEA-ST may play an important role in the excretion of endogenous and exogenous compounds.
...
PMID:Investigation of the localization of dehydroepiandrosterone sulphotransferase in adult rat kidney. 1101 88
