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Query: EC:2.7.7.48 (
transcriptase
)
9,479
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Metagenomics has altered our understanding of microbial diversity and ecology. This includes its applications to viruses in marine environments that have demonstrated their enormous diversity. Within these are RNA viruses, many of which share genetic features with members of the order
Picornavirales
; yet, very few of these have been taxonomically classified. The only recognized family of marine RNA viruses is the
Marnaviridae
, which was founded based on discovery and characterization of the species
Heterosigma akashiwo RNA virus
. Two additional genera of marine RNA viruses,
Labyrnavirus
(one species) and
Bacillarnavirus
(three species), were subsequently defined within the order
Picornavirales
but not assigned to a family. We have defined a sequence-based framework for taxonomic classification of twenty marine RNA viruses into the family
Marnaviridae
. Using
RNA-dependent RNA polymerase
(RdRp) phylogeny and distance-based analyses, we assigned the genera
Labyrnavirus
and
Bacillarnavirus
to the family
Marnaviridae
and created four additional genera in the family:
Locarnavirus
(four species),
Kusarnavirus
(one species),
Salisharnavirus
(four species) and
Sogarnavirus
(six species). We used pairwise capsid protein comparisons to delineate species within families, with 75 per cent identity as the species demarcation threshold. The family displays high sequence diversities and Jukes-
Cantor
distances for both the RdRp and capsid genes, suggesting that the classified viruses are not representative of all of the virus diversity within the family and that there are many more extant taxa. Our proposed taxonomic framework provides a sound classification system for this group of viruses that will have broadly applicable principles for other viral groups. It is based on sequence data alone and provides a robust taxonomic framework to include viruses discovered via metagenomic studies, thereby greatly expanding the realm of viruses subject to taxonomic classification.
...
PMID:Application of a sequence-based taxonomic classification method to uncultured and unclassified marine single-stranded RNA viruses in the order
Picornavirales
. 3190 48
