Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.7.48 (
transcriptase
)
9,479
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The expression of osteopontin (OPN) was studied in the brains of mice with
scrapie
. Reverse
transcriptase
polymerase chain reaction (RT-PCR) and Western blot analysis showed that the expression of OPN protein and mRNA was increased significantly in the
scrapie
-infected brains compared to the controls. The increased expression of OPN protein was largely matched with the PrP(Sc) accumulation. Immunohistochemically, OPN was intensely immunostained in neurons of the midbrain at the time of
scrapie
infection initiation. Particularly, OPN immunostaining was noted in the reactive astrocytes and some microglia in the
scrapie
brains, while those cells were devoid of OPN immunoreactivity in control brains. Overall, these findings suggest that some neurons affected by PrP(Sc) at an early stage of
scrapie
transiently express OPN but subsequently succumb to cell death at a later stage of
scrapie
; astroglial cells after
scrapie
infection are activated to express OPN; and increased OPN expression in these cells may play an important role in the pathology of
scrapie
. The precise role of OPN in
scrapie
needs further study.
...
PMID:Increased expression of osteopontin in the brain with scrapie infection. 1641 98
