Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:2.7.7.48 (transcriptase)
 9,479 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

MSU-TnT4 (TnT4) cells, a newly established Trichoplusia ni cell line, was examined for the presence of a latent nodavirus, as had been described for another T. ni cell line, BTI-TN-5B1-4 (Hi5) cells. Reverse transcriptase polymerase chain reaction using nodavirus-specific primers did not detect virus genomic RNA in TnT4 cells. Transmission electron microscopy of recombinant baculovirus-infected TnT4 cells showed no evidence of latent nodavirus activation. Nodavirus particles were not detected in density gradients of baculovirus-infected TnT4 cell lysates or cell supernatants. The same methods confirmed the presence of a latent nodavirus in Hi5 cells.
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PMID:The Trichoplusia ni cell line MSU-TnT4 does not harbor a latent nodavirus. 1991 Dec 41

In an epidemiological study of ferret coronaviruses (FRCoVs), novel FRCoV strains (Saitama-1 and Aichi-1) were detected by reverse transcription-polymerase chain reaction (RT-PCR) and nucleotide sequence analysis of partial RNA-dependent RNA polymerase (RdRp) genes. Phylogenetic analysis indicated that these strains belonged to different clusters from other FRCoV strains. Next, the nucleotide sequence of the 3'-terminal region of Saitama-1 (8271 bases) strain was determined and compared with those of the other FRCoVs, indicating that the Saitama-1 strain differed from the previously reported MSU-1 and MSU-2 strains in the regions encoding spike (S) protein, nucleocapsid, and open reading frame 7b. Furthermore, the results of SimPlot analysis indicated that FRCoV (MSU-2 strain) emerged via a recombination event of S protein between the MSU-1 and Saitama-1 strains. This mechanism is similar to that responsible for the emergence of type II feline coronavirus. This information will be useful for understanding the pathogenesis of FRCoV in ferrets.
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PMID:Detection of novel ferret coronaviruses and evidence of recombination among ferret coronaviruses. 2736 29