Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.13.3 (
histidine kinase
)
2,405
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The
EnvZ
-OmpR histidyl-aspartyl phosphorelay system in E. coli responds to osmolarity by differentially modulating the expression of the major outer membrane porins OmpF and OmpC. To date, the natural ligand that activates
EnvZ
, a transmembrane
histidine kinase
, has not been identified and the role of the periplasmic domain of
EnvZ
is unclear. We now report on the purification and characterization of the periplasmic domain of
EnvZ
(Lys48-Arg162) which has been expressed as a soluble protein in fusion with the maltose-binding protein. Overexpression of the fusion protein did not compete for a signal that activates
EnvZ
. By amylose affinity chromatography and affinity blotting, interacting proteins could not be detected. The periplasmic domain was released by
factor Xa
and purified to homogeneity. From circular dichroism analysis, the periplasmic domain was estimated to consist of 35% alpha-helices and 16% beta-sheets.
...
PMID:Purification and characterization of the periplasmic domain of EnvZ osmosensor in Escherichia coli. 907 Feb 21
