Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.13.3 (
histidine kinase
)
2,405
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The heme-based oxygen sensor
histidine kinase
Af
GcHK is part of a two-component signal transduction system in bacteria. O
2
binding to the Fe(II) heme complex of its N-terminal globin domain strongly stimulates autophosphorylation at His
183
in its C-terminal kinase domain. The 6-coordinate heme Fe(III)-OH
-
and -CN
-
complexes of
Af
GcHK are also active, but the 5-coordinate heme Fe(II) complex and the heme-free apo-form are inactive. Here, we determined the crystal structures of the isolated dimeric globin domains of the active Fe(III)-CN
-
and inactive 5-coordinate Fe(II) forms, revealing striking structural differences on the heme-proximal side of the globin domain. Using hydrogen/deuterium exchange coupled with mass spectrometry to characterize the conformations of the active and inactive forms of full-length
Af
GcHK in solution, we investigated the intramolecular signal transduction mechanisms. Major differences between the active and inactive forms were observed on the heme-proximal side (helix H5), at the dimerization interface (helices H6 and H7 and loop L7) of the globin domain and in the ATP-binding site (helices H9 and
H11
) of the kinase domain. Moreover, separation of the sensor and kinase domains, which deactivates catalysis, increased the solvent exposure of the globin domain-dimerization interface (helix H6) as well as the flexibility and solvent exposure of helix
H11
. Together, these results suggest that structural changes at the heme-proximal side, the globin domain-dimerization interface, and the ATP-binding site are important in the signal transduction mechanism of
Af
GcHK. We conclude that
Af
GcHK functions as an ensemble of molecules sampling at least two conformational states.
...
PMID:Coordination and redox state-dependent structural changes of the heme-based oxygen sensor
Af
GcHK associated with intraprotein signal transduction. 2909 8
