Gene/Protein
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Symptom
Drug
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Pivot Concepts:
Gene/Protein
Disease
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Drug
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Target Concepts:
Gene/Protein
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Query: EC:2.7.12.2 (
MEK
)
18,161
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The symbiosis receptor kinase, SymRK, is required for root nodule development. A SymRK-interacting protein (
SIP2
) was found to form protein complex with SymRK in vitro and in planta. The interaction between SymRK and
SIP2
is conserved in legumes. The
SIP2
gene was expressed in all Lotus japonicus tissues examined.
SIP2
represents a typical plant
mitogen-activated protein kinase kinase
(
MAPKK
) and exhibited autophosphorylation and transphosphorylation activities. Recombinant
SIP2
protein could phosphorylate casein and the Arabidopsis thaliana MAP kinase MPK6. SymRK and
SIP2
could not use one another as a substrate for phosphorylation. Instead, SymRK acted as an inhibitor of
SIP2
kinase when MPK6 was used as a substrate, suggesting that SymRK may serve as a negative regulator of the
SIP2
signaling pathway. Knockdown expression of
SIP2
via RNA interference (RNAi) resulted in drastic reduction of nodules formed in transgenic hairy roots. A significant portion of
SIP2
RNAi hairy roots failed to form a nodule. In these roots, the expression levels of
SIP2
and three marker genes for infection thread and nodule primordium formation were downregulated drastically, while the expression of two other
MAPKK
genes were not altered. These observations demonstrate an essential role of
SIP2
in the early symbiosis signaling and nodule organogenesis.
...
PMID:A MAP kinase kinase interacts with SymRK and regulates nodule organogenesis in Lotus japonicus. 2235 70
Symbiosis receptor-like kinase (SymRK) is a key protein mediating the legume-Rhizobium symbiosis. Our previous work has identified an
MAP kinase kinase
,
SIP2
, as a SymRK-interacting protein to positively regulate nodule organogenesis in Lotus japonicus, suggesting that an MAPK cascade might be involved in Rhizobium-legume symbiosis. In this study, LjMPK6 was identified as a phosphorylation target of
SIP2
. Stable transgenic L. japonicus with RNAi silencing of LjMPK6 decreased the numbers of nodule primordia (NP) and nodule, while plants overexpressing LjMPK6 increased the numbers of nodule, infection threads (ITs), and NP, indicating that LjMPK6 plays a positive role in nodulation. LjMPK6 could interact with a cytokinin receptor, LHK1 both in vivo and in vitro. LjMPK6 was shown to compete with LHP1 to bind to the receiver domain (RD) of LHK1and to downregulate the expression of two LjACS (1-aminocyclopropane-1-carboxylic acid synthase) genes and ethylene levels during nodulation. This study demonstrated an important role of LjMPK6 in regulation of nodule organogenesis and ethylene production in L. japonicus.
...
PMID:An MAP kinase interacts with LHK1 and regulates nodule organogenesis in Lotus japonicus. 3069 31
