Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:2.7.11.8 (FAST)
 758 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Not much data is available on the reliability diagnosis of IgE FAST, in comparison with other "in vitro" and "in vivo" techniques. The motive of this study was to evaluate FAST sensitivity in patients with rhinitic allergies, comparing it with RAST and the prick test. Thirty-four patients, monosensitized in 11 cases for pollen, in 16 for mites and in 7 for moulds were studied. Ten patients with negative nasal provocation were used for the control group. In a total of 133 sera, there was class concordance between FAST and prick test in 59.39% of the cases, with a r = +0.41 and p less than 0.001 correlation coefficient. Corcordance of FAST and RAST for a total of 135 sera was 65.18%. Concordance in those who had nasal positive provocation was 87.65% for FAST, 75.04% for RAST and 92.85% for prick. From our experience with FAST, in patients with allergic rhinitis for the pneumoallergens evaluated, we conclude that this test constitutes an alternative "in vitro" diagnosis in the cases indicated. We found that its advantages were its low cost in the way of human and economical resources, its greater reliability in comparison with RAST, its simple administration and it being harmiess.
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PMID:Reliability diagnosis of FAST in allergic rhinitis. Correlation with RAST, nasal provocation and prick test. 328 32

This reports covers the application to paediatrics of total IgE determination by the means of a new direct, sandwich type, fluorescence enzyme immuno-assay (Fluoro-allergo sorbent test:FAST IgE). The technical process is adapted for usual determinations, in case of infants and young children, of low normal rates with a good sensitivity, and of high pathological rates. IgE total usual values are established by the FAST IgE test from serums of healthy children, aged from one day to 14 years. Seven age groups are to be distinguished.
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PMID:[Total serum IgE: normal pediatric values using the FAST IgE fluoroimmunoenzymatic technic]. 333 Nov 7

The IgG4 is the predominant antibody response in patients receiving chronic exposure to high doses of antigen, and this seems to be true for immunotherapy as well. The duration and the dose of immunotherapy (IT) seems to increase specific IgG4 levels, but this increase does not seem to be related to the clinical response, seasonal exposure, duration of disease, and IgE antibody levels. The measurement of IgE and IgG4 antibodies to allergens did not predict the clinical effect of immunotherapy in our study. However, it indicated that the patients were continuing to receive the allergenic extracts and that the extract used in vivo, although produced by a different manufacturer, was closely resembling the one used for coating plastic wells in the FAST assay. The increase of antibody titres demonstrate that long term monitoring of IT administration may be accomplished by these in vitro tests, although individual patients may exhibit different responses in time but similar clinical results. Longitudinal studies on a cohort of atopic subjects may help define more precisely doses and timings required to achieve useful indications on both compliance with IT injections and predictivity of its outcome.
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PMID:IgE and IgG4 levels in allergic patients during immunotherapy. 348 27

The FAST assay (Allergenetics) for the determination of specific IgE has recently been introduced. The results of this test, graded in classes 0 (negative), 1 (equivocal) and 2 to 6 (positive) were compared with those of another commercially available test (Phadezym RAST, Pharmacia), graded from class 0 (negative) and classes 1 to 4 (positive). In 52 adults suffering from rhinitis/asthma, a total of 114 positive skin prick tests (SPT) were obtained for common respiratory allergens. In 67% of these tests the Phadezym RAST was positive and in 63% the FAST was positive (classes 2 to 6). In these patients there were 151 negative SPT: 6% corresponded to a positive Phadezym RAST (all class 1) and 34% to a positive FAST (classes 2 to 6). The serum of nine nonatopic volunteers who had negative SPT for 12 common allergens were tested. In none did the Phadezym RAST give any positive results; the FAST was positive in all nine sera, detecting between five and 11 allergens. When both assays were performed on 14 unselected cord blood samples, the Phadezym RAST was positive in three samples (with class 1 results to a maximum of two allergens), and the FAST was positive in 12 samples, detecting between one and seven allergens. Thus, in our hands, the FAST gave an abnormally high number of positive results in patients with negative SPT, in nonatopic volunteers, and in cord blood.
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PMID:Comparison of two assays for the determination of specific IgE in serum of atopic and nonatopic subjects: the Allergenetics FAST and the Phadezym RAST. 375 16

Prick skin testing and IgE FASTTM results were compared in eight atopic patients, using a comparable class system. Identical results were found in 53.5% of the cases; a total of 85.7% of the results were within one class difference. Sera from 436 patients were assayed for specific IgE to 94 allergens for a total of 436 test comparisons using IgE FASTTM and modified RAST. Identical results were found in 41.7% of cases; a total of 81.4% of the tests differed by one class or less. The IgE FAST showed good correlation both with prick testing and modified RAST. IgE FAST appears to be a valid non-radioactive, time-efficient method for detecting specific IgE to common inhalant and environmental allergens.
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PMID:Correlation of allergy test results obtained by IgE FAST, RAST, and prick-puncture methods. 388 Oct 59

It is possible to treat vertebral disc hernias by chemonucleolysis because of the enzymatic properties of chymopapain extracted from Carica papaya. But, 1% of the general population would seem to have a latent sensitivity to this protein, and would thus be at risk of presenting life-threatening anaphylactic shock. Recent clinical studies have identified different risk factors: atopy, previous food and drug allergies. A case is here reported of a 35 year old woman with a history of urticaria following anti-tetanus serum and penicillin injections, who frequently ate exotic fruit, and who was intolerant to alcohol. HBDT and prick tests confirmed both drug allergies. A prick test to chymopapain 1 mg X ml-1 gave a borderline result; the HBDT was positive, with 45% degranulation. Both these tests had been previously assessed by a study of 20 volunteers in good general health: negative prick tests in all 20, and negative HBDT in 19 out of the 20, with chymopapain concentrations ranging from 10 micrograms X ml-1 to 1 micrograms X ml-1. The one volunteer with a positive HBDT probably had latent sensitivity to the enzyme. The great sensitivity of both prick tests and HBDT in detecting IgE specific for food proteins is recalled. It is suggested that a routine predictive immuno-allergological assessment be carried out, with prick tests to the standard airbone allergens (to find a possible atopy), and a prick-test with 1 mg X ml-1 chymopapain, and a HBDT to the enzyme. A sample of serum should be kept for possible RAST and FAST carried out later.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:[Allergy to chymopapain: value of predictive tests before chemonucleolysis]. 389 37

An IgE FAST(TM) inhibition protocol has been developed as a rapid in vitro method for determining relative potencies of in vivo allergenic extracts. Results are obtained within one day and correlate well with those of the RAST inhibition protocol (r = .96), making the FAST assay a preferable procedure for inprocess potency determination during the manufacture of standardized allergenic extracts.
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PMID:IgE FAST inhibition: a rapid technique for the standardization of pollen allergen extract potency. 395 62

The study compares results of skin test and assessments of venom specific IgE levels (FAST) in patients with Hymenoptera sting allergic reactions. Results of the two diagnostic methods show considerable correlation. Positive correlation occurred in 90% of the patients (92.4% bee venom sensitive allergic patients and 80% wasp venom sensitive patients). Negative correlation occurred in 2% of the patients. In the remaining 8% of the group the results did not correlate. Basophil histamine release test was performed in 13 patients with allergic reactions after stinging. 77% of the basophil histamine release test results showed positive correlation with the other two tests. No correlation was found between the size skin test, the class of the FAST test, and the result of basophil histamine release test. No relationship was found either, between results of the described test and the severity of sting reaction classified to Mueller scale.
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PMID:[Evaluation of diagnostic value of skin test, venom specific antibodies against EgE and basophil histamine release test in hymenoptera allergy]. 769 38

The value of IgG4 determination in food allergy shows conflicting results in the medical literature. Our pediatric study was carried out in two phases. The first compared the percentage of detection and agreement of specific IgE (RAST) and IgG4 (FAST) for egg white and egg yolk in 104 patients with possible hypersensitivity to egg and positive skin tests. The second, in 22 new patients, compared IgG4 determination with the oral provocation test (OPT; the diagnostic "gold standard"). In the first phase, the percentage of positive cases for both antibodies was almost identical, but little agreement was observed between them. In the second phase, the sensitivity, specificity and agreement with the OPT were higher for IgE than for IgG4. Thus, we conclude that IgG4 levels may be high in food allergy, but their detection seems to have little diagnostic value in allergy to egg proteins in children.
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PMID:The value of specific IgG4 determination in childhood allergy to egg in relation to specific IgE and the provocation test. 792 32

The study comprised of 50 patients with the diagnosis of pollinosis based on characteristic history data, positive results of skin test and positive IgE FAST test with grass pollen allergens. 20 ml of blood was taken from all patients and total E rosette test was carried. Antihistamine was added to rosette test in the concentration of 10(-3) mg/ml in the following doses: 0.1 ml, 0.2 ml, 0.3 ml. Histamine caused stat. sig. (P < 0.001) decrease in the percentage of lymphocytes forming E rosettes. We think that lymphocyte may be injured by histamine under exposition to grass pollens.
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PMID:[Effect of histamine on behavior of the rosette test in patients with pollinosis]. 795 Oct 80


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