Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound   Target Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound

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Query: EC:2.7.11.8 (FAST)
758 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Six subjects (4 female, 2 male), aged from 16 to 25 years, presented with allergic rhinitis to Dermatophagoides mites and received SIT by the sub-cutaneous route with delayed-release alpha fraction Bayropharm at the standard doses. Diagnosis was based on clinical history, skin tests and measurement of specific IgE at 0, 3, 9, and 12 months, by the fluoro-enzymatic technique (FAST). For comparison, in a reference group (n = 20) the IgE varied between 0.32 and 0.11 IU/ml for D1 and 0.31 to 0.09 IU/ml for D2. The eight patients had specific IgE titres of D1 = 0.96, D2 = 0.99. For these authors, the FAST technique used for the measurement of specific IgE, although less sensitive than the RIA technique of RAST, gives a good evaluation of SIT.
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PMID:IgE modification of the specific antidermatophagoides during the first year of specific immunotherapy (SIT). 128 44

The measurement of IgE and IgG4 antibodies against egg white, milk, soybean and Dermatophagoides farinae was performed by FAST (fluorescence allergosorbent test) using 21 serum samples obtained from non-allergic children and 160 serum samples from atopic children with bronchial asthma and/or atopic dermatitis. Their antibody levels were evaluated for any association with disease severity and for clinical significance in establishing diagnosis. It was found that children with bronchial asthma showed lower levels of IgE antibodies against egg white, milk and soybean and higher levels of IgE antibodies against Dermatophagoides farinae compared with those of children with atopic dermatitis, while both groups showed higher levels of egg white and milk-specific IgG4 antibodies compared with non-allergic children. These IgE and IgG4 antibody levels revealed a tendency to correlate with disease severity in patients with atopic dermatitis, while this was not observed in patients with bronchial asthma. The contribution percentages of IgG4 antibody determination, together with IgE antibody determination, in retrieving causal allergens were 71% for egg white, 70% for milk and 48% for soybean allergy, implying their diagnostic value in establishing clinical diagnosis.
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PMID:[Clinical significance of IgG4 antibody determination in children against egg white, milk, soybean and Dermatophagoides farinae]. 129 Apr 13

Irrelevant IgE binding to cellulose discs is known to give false positive results in Phadezym RAST (Pharmacia) for the estimation of allergen-specific IgE in serum. We investigated FAST-Plus Test (3M Diagnostic Systems), an enzyme-linked sandwich type Fluoro-Allergo-Sorbent Test in which a particular allergen was coated to polystyrene well. Phadezym RAST and CAP RAST (Pharmacia) using cellulose-derivative discs as adsorbent were used as reference methods. Patients' sera which gave negative blank reactions to uncoated filter paper disc in the Phadezym RAST system were assayed for specific IgE to 6 allergens using FAST-Plus Test, CAP RAST and Phadezym RAST, and the results of the former two were compared with those of Phadezym RAST using a comparable class system. FAST-Plus Test showed variable correlations with Phadezym RAST, the correlation coefficients ranged from 0.41 to 0.97 (r = 0.462 in house dust 1, r = 0.713 in house dust 2, r = 0.412 in Candida albicans, r = 0.952 in Dermatophagoides peteronyssinus, r = 0.969 in Dermatophagoides farinae and r = 0.682 in Japanese cedar), although most of the results were within one class difference. Similar correlations were obtained between CAP RAST and Phadezym RAST. Of 3004 patients' sera tested in the past two years using Phadezym RAST, 132 (96 cases) displayed positive blank reactions to the uncoated filter paper disc. Of the 96 cases, 80 sera were assayed for binding of IgE to the uncoated cellulose-derivative disc in the CAP RAST system. 18 showed positive results up to 7 IU/ml.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:[False positive reaction in measurement of allergen-specific IgE--comparison of 3M IgE FAST-Plus Test using polystyrene well as adsorbent with Phadezym RAST]. 130 29

IgG4-anti-IgE antibodies have been identified in normal nonatopic subjects, as well as in allergic patients. Their potential role remains controversial. We studied 44 Chinese children with allergic symptoms, and measured their IgG4-anti-IgE serum levels using a modified IgG4 FAST assay. The levels of IgG4-anti-IgE antibodies did not correlate with clinical status. The usefulness and relevance of these measurements remain questionable in allergic patients.
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PMID:IgG4-anti-IgE antibodies in Chinese allergic children. 141 58

Since differences in HLA groups have been found in Chinese populations, as well as differences in the values of total IgE in China, Japan and Thailand, a study has been done in 44 Chinese children, all of them with a history of atopic conditions divided into five groups: asthma, rhinitis, conjunctivitis, allergic bronchitis and atopic dermatitis. 34 of them were positive against D. pteronyssimus, 8 against cats, 3 against dogs and 3 against fish. The only adult patient studied was positive against gramineal plants (all positivities were tested with the prick test). IVT ELISA Screen is performed against 10 allergens, as well as 3 M FAST-Test and 3 M Total IgE FAST Plus Test. The results show that the majority of children had an elevation of the total IgE in the three tests. There is quite a good agreement between FAST and IgE-IVT and a better correlation between IgE-IVT and IgE FAST Plus. The correlation between IgE FAST and IgE FAST Plus was excellent.
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PMID:Evaluation of serum total IgE in Chinese allergic children using different assays. 144 49

The measurement of IgE antibodies and total IgE was performed with FAST (fluorescene allergosrobent test) system using 171 serum samples obtained from normal donors and 789 serum samples obtained from patients with various allergic diseases. These were collected by departments of internal medicine, pediatrics, otorhinolaryngology and dermatology at 17 institutes in Japan. In addition to a comparative study with RAST, skin tests and provocation tests were also performed to establish a clinical diagnosis. Simultaneous measurements were made and an excellent correlation with RAST was observed with a concordance rate of 85.7% and correlation coefficient of 0.848. The specificity determined in the normal serum samples was 95.5% by RAST and 93.1% by FAST. The sensitivity determined in the samples, of which etiological allergens were identified clinically, was 82.3% by RAST and 85.3% by FAST. Among them, the sensitivity of skin test and provocation test were 92.4% and 94.7%, respectively. The concordance rate with clinical diagnosis was as high as 89.7% for both RAST and FAST. The normal upper limit of total IgE by cumulative 95% value was calculated to be 250 IU/ml for adult subjects. These results indicated the clinical usefulness of the FAST system in evaluating IgE antibodies and total IgE.
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PMID:[Evaluation of allergen-specific IgE antibody and total IgE with a new IgE detection system named FAST: fluorescence allergosorbent test]. 156 87

The significance of "borderline" levels of allergen-specific IgE as measured by in vitro assays has been questioned. Patients whose specific IgE tests Patients were tested for twelve antigens using the FAST-Plus methodology. All 0/1 results were checked using skin tests at a 1:500 concentration. Positive (histamine) and negative (diluent) controls were used. The antigen-induced wheals were compared with those produced by a control wheal of 2% glycerine (the glycerine concentration in a 1:500 dilution). Positive wheals were arbitrarily considered to be those whose diameter after 10 minutes exceeded that of the glycerine control wheal by 2 mm or more. Using the limits of calibrator fluorescence for the FAST-Plus test in effect before 1990, a significant discordance between skin test results and the class 0/1 in vitro readings was evident. Using the standards in effect since 1990, marked concordance between class 0/1 results and positive skin tests was noted. This was most marked for pollens, less so for molds. Using current standards, FAST-Plus class 0/1 results are best considered positive (pending clinical confirmation), rather than negative.
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PMID:Significance of borderline levels of specific IgE obtained by FAST-Plus assay. 158 16

In 25 subjects with pollinosis the following parameters were assessed three times annually (early spring, pollen seasons, autumn): serum IgE levels and allergen specific IgE (asIgE), T lymphocyte subpopulation in peripheral blood. In selected patients allergen specific IgG-4 levels were calculated. All serum parameters were assessed using the FAST enzymatic methods (3M Diagnostic Systems). Lymphocyte subpopulations were assessed using the monoclonal antibodies (Ortho Diagnostic System). During the pollen seasons a statistically significant increase of serum IgE, asIgE and T suppressor cells was found. The CD4/CD8 ratio during this period was decreased, caused by stable level of T helper cells. In the 4 patients the levels of asIgG-4 did not change although the initial levels were elevated.
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PMID:[Seasonal changes in various immunological parameters in patients with hay fever]. 184 5

The study involved 117 adults and 535 children with atopic dermatitis. Immunoglobulins E against D. pteronyssinus--main allergen of the home dust--were assayed with RAST technique in children and FAST technique in adult patients. It was found that the blood serum IgE levels increase with patients' age and is the highest in patients with coexisting allergic respiratory diseases (difference statistically significant). RAST precision was compared with that of "prick" skin tests in the detection of allergy to home dust mites. Desensitization of 15 patients with home mite allergen produced satisfactory effects.
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PMID:[Sensitivity to the primary house dust allergen--dermatophagoides pteronyssinus--in patients with atopic dermatitis]. 209 37

The accuracy of measurement using kits in the clinical laboratories is important for the patient diagnosis and treatment. In the present paper, the AL-18, AlaSTAT, CAP, FAST and RAST methods were investigated and were compared among kits the results obtained with serum sample, for determination of specific IgE antibodies. Significant differences among kits were observed from the results of those methods. One of the reasons, why the data discrepancy exists, is that each kit uses a different reference and a different inclusion method of allergen. For the evaluation of data discrepancy among those kits, it might be important that the clinical history of symptoms and in vivo tests against the different allergens compared with results of in vivo tests.
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PMID:[A comparison of the several methods for determination of specific IgE antibodies]. 224 59


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