Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.11.8 (
FAST
)
758
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Yellow Fluorescence-Activating and absorption-Shifting Tag (Y-
FAST
, hereafter called
FAST
) is a
14 kDa protein
tag giving a bright green-yellow fluorescent complex upon interaction with the fluorogenic dye 4-hydroxy-3-methylbenzylidene rhodanine (HMBR). Here, we report a collection of fluorogens enabling tuning of the fluorescence color of
FAST
from green-yellow to orange and red. Beyond allowing the multicolor imaging of
FAST
-tagged proteins in live cells, these fluorogens enable dynamic color switching because of
FAST
's reversible labeling. This unprecedented behavior allows for selective detection of
FAST
-tagged proteins in cells expressing both green and red fluorescent species through two-color cross-correlation, opening up exciting prospects to overcome spectral crowding and push the frontiers of multiplexed imaging.
...
PMID:Dynamic multicolor protein labeling in living cells. 2897 Sep 39
