Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:2.7.11.8 (FAST)
 758 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Because end-organ injury can occur with reperfusion following hemorrhage or ischemia, we hypothesized that aggressive intravenous fluid resuscitation would aggravate tissue injury in a fixed-volume model of hemorrhagic shock. Unanesthetized chronically prepared male rats were hemorrhaged 33-36 mL/kg for 2.5 h. Then Lactated Ringers Solution (3x hemorrhage volume) was infused over 5 min (FAST), 20 min (MEDIUM), 180 min (SLOW), or not at all (NO RESUS). Plasma ornithine carbamoyltransferase (OCT), lactate, and creatinine were measured as indices of hepatocellular injury, anaerobic metabolism, and renal function, respectively. At 1 h post-resuscitation (PR), MAP was greater after SLOW and MEDIUM treatment (tx) than after other txs (P < 0.05). OCT increased earliest after FAST tx to values greater than those after other txs from 30 min to 24 h PR (P < 0.01). Plasma lactate was elevated immediately before resuscitation in all groups (P < 0.01) and returned to baseline at 3 h PR after SLOW tx compared to 5 h PR after FAST tx (P < 0.05). Creatinine at 5 h PR was less in the groups treated with intravenous fluid compared to the NO RESUS group, P < 0.05. Survival at 72 h was reduced in the FAST (57%) and NO RESUS (58%) groups compared to the SLOW (87%) and MEDIUM (85%) groups (P < 0.05). Thus, overly aggressive fluid tx accelerates hepatocellular injury, is no better than lesser rates of resuscitation at correcting plasma lactate and preserving renal function, and provides no overall survival benefit.
 ...
PMID:Detrimental effects of rapid fluid resuscitation on hepatocellular function and survival after hemorrhagic shock. 1235 25

Myxomatous mitral valves (MVs) contain elevated proportions of unique cell populations such as myofibroblasts. Without a reliable technique to isolate such cell populations, however, it has been difficult to study the role of these cells. The goal of this study was to use fibronectin (FN) to isolate distinct cell subpopulations from normal porcine MVs. Cells from porcine posterior MV leaflets were separated based on time-dependent adhesion to either tissue culture plastic (TCP) flasks or FN-coated flasks. The resultant "FAST" and "SLOW" adhering subpopulations from each technique were phenotyped using flow cytometry and immunocytochemistry to detect expression of myofibroblast markers, enzymes for collagen synthesis, and MAP kinases. Compared with FN SLOW, FN FAST showed significantly higher expression of prolyl 4-hydroxylase, heat shock protein-47 (HSP47), smooth muscle alpha-actin (SMalphaA), nonmuscle myosin (Smem), extracellular-related signaling kinase (ERK) 1, ERK2, and phosphorylated-ERK. In contrast, TCP FAST showed higher expression of only HSP47, SMalphaA, and Smem compared with TCP SLOW. In conclusion, differential adhesion to FN successfully separated a myofibroblast-like subpopulation from the posterior leaflet of the MV. This subpopulation may be useful in studying myxomatous MV disease, although additional studies remain to verify that this myofibroblast-like population resembles that observed in myxomatous MV disease.
 ...
PMID:Fibronectin-based isolation of valve interstitial cell subpopulations: relevance to valve disease. 1918 92