Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:2.7.11.8 (FAST)
 758 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

On 132 patients with case history of rhinitis and/or asthma in a domestic environment and with positive cutaneous tests contrasted with D. pteronyssinus, we have made cutaneous tests compared to said antigen and compared with D. farinae, L. destructor and A. siro, with extracts 5000 E/ml (Noon Units), as well as FAST inhibition, using D. pteronyssinus in its solid phase and all the above mentioned extracts in their liquid phase. Our results show that the frequency of D. pteronyssinus positivities in prick is greater than the other mites as well as the intensity of the response expressed in the size of the wheal showing significant differences between those found for D. pteronyssinus and those obtained with other mites. In turn those obtained with the D. farinae are significantly greater than those obtained with non-pyroglyphide mites. In FAST inhibition we only found a good correlation for D. pteronyssinus-D. pteronyssinus and D. pteronyssinus-D. farinae and there is no good correlation between the other mites. The correlation coefficients differ significantly except for those of the pyroglyphides, and the regression equations show a lack of parallelism in the regression lines. Consequently, on the basis of this study, we conclude that there is no crossed reactivity between D. peteronyssinus and non-pyroglyphide mites.
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PMID:Study of the possible crossed reactivity between D. pteronyssinus and non pyroglyphide acarus. 1. Skin tests and FAST inhibition. 148 96

The significance of "borderline" levels of allergen-specific IgE as measured by in vitro assays has been questioned. Patients whose specific IgE tests Patients were tested for twelve antigens using the FAST-Plus methodology. All 0/1 results were checked using skin tests at a 1:500 concentration. Positive (histamine) and negative (diluent) controls were used. The antigen-induced wheals were compared with those produced by a control wheal of 2% glycerine (the glycerine concentration in a 1:500 dilution). Positive wheals were arbitrarily considered to be those whose diameter after 10 minutes exceeded that of the glycerine control wheal by 2 mm or more. Using the limits of calibrator fluorescence for the FAST-Plus test in effect before 1990, a significant discordance between skin test results and the class 0/1 in vitro readings was evident. Using the standards in effect since 1990, marked concordance between class 0/1 results and positive skin tests was noted. This was most marked for pollens, less so for molds. Using current standards, FAST-Plus class 0/1 results are best considered positive (pending clinical confirmation), rather than negative.
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PMID:Significance of borderline levels of specific IgE obtained by FAST-Plus assay. 158 16

The purpose of this study is to assess the frequency of clinical sensitivity to Eastern White Pine Pollen, 100 consecutive patients with the seasonal (SAR) or perennial (PAR) allergic rhinitis seen in the allergy clinic were prick tested with pine pollen extract, 8-tree mixture, histamine and negative control. Positive skin test (ST) was defined as a wheal greater than 3 mm larger than control, plus flare. Patients with a positive ST were then asked to stop antihistamine and other related drug, for 48 hours and challenged in a double blind manner with increasing concentrations of intranasal pine extract, starting at 1/100,000 w/v, followed by 1/10,000, 1/1000 and 1/100 at 20 minute intervals. The dose given was 0.15 cc by metered dose spray; one nostril received pine extract diluted in saline, the other received plain saline,. Rhinometric measurements were obtained before and 20 minutes after each challenge. Positive challenges were defined as 1) subjective feeling of increased stuffiness or rhinorrhea and 2) greater than 25% decrease in nasal airflow. Six patients (6%) had a positive ST to pine pollen extract and two of four patients with positive pine skin test had a positive FAST. Four of these were challenged intranasally, 2 had a positive challenge. All six patients had a history of spring SAR and positive reaction to 8-tree mix. Out of the 100 patients skin tested, 61 had spring SAR; therefore, the incidence of positive ST to pine in patients with spring SAR was 6/61 (10%). We conclude that pine pollen can be a cause of spring SAR in the New England area.
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PMID:Eastern pine sensitivity in New England. 341 92