Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:2.7.11.8 (FAST)
 758 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We have successfully established a novel protein microarray-based kinase assay, which we applied to identify target proteins of the barley protein kinase CK2alpha. As a source of recombinant barley proteins we cloned cDNAs specific for filial tissues of developing barley seeds into an E. coli expression vector. By using robot technology, 21,500 library clones were arrayed in microtiter plates and gridded onto high-density filters. Protein expressing clones were detected using an anti-RGS-His6 antibody and rearrayed into a sublibrary of 4100 clones. All of these clones were sequenced from the 5'-end and the sequences were analysed by homology searches against protein databases. Based on these results we selected 768 clones expressing different barley proteins for protein purification. The purified proteins were robotically arrayed onto FAST slides. The generated protein microarrays were incubated with an expression library-derived barley CK2alpha in the presence of [gamma-33P]ATP, and signals were detected by X-ray film or phosphor imager. We were able to demonstrate the power of the protein microarray technology by identification of 21 potential targets out of 768 proteins including such well-known substrates of CK2alpha as high mobility group proteins and calreticulin.
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PMID:Identification of barley CK2alpha targets by using the protein microarray technology. 1527 36

The assessment of malingering is a fundamental component of forensic evaluations that should be considered with each referral. In systematizing the evaluation of malingering, one option is the standardized administration of screens as an initial step. The current study assessed the effectiveness of three common screening measures: the Miller Forensic Assessment of Symptoms Test (M-FAST; Miller, 2001), the Structured Inventory of Malingered Symptomatology (SIMS; Widows & Smith, 2004), and the Evaluation of Competency to Stand Trial-Revised Atypical Presentation Scale (ECST-R ATP; Rogers, Tillbrook, & Sewell, 2004). Using the Structured Interview of Reported Symptoms (SIRS) as the external criterion, 100 patients involved in competency to stand trial evaluations were categorized as either probable malingerers (n=21) or nonmalingerers (n=79). Each malingering scale produced robust effect sizes in this known-groups comparison. Results are discussed in relation to the comprehensive assessment of malingering within a forensic context.
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PMID:An evaluation of malingering screens with competency to stand trial patients: a known-groups comparison. 1705 21

Left ventricular mechanical dyssynchrony from right ventricular apical (RVA) pacing may lead to heart failure. Mitochondrial dysfunction has been observed in the failing heart; however, whether RVA pacing may alter the underlying mitochondrial dynamics at an early stage in patients with preserved ejection fraction is not well understood. RNA was isolated from peripheral whole blood samples of 13 patients. The differentially expressed mRNA profiles from 58 samples (13 experimental subjects; 35 control subjects) were performed using Affymetrix array. Finally, a five-gene signature was identified. DAVID was performed to explore the biological functions of target genes with altered gene expression between two groups. The gene signature (OPA1, CTSA, NDUFA1, STK10 and PRDX1) was able to identify patients post-implant with an area under receiver operating characteristic curve of 0.90 in this study. Our test showed that the gene signature had a sensitivity of 91% with a specificity of 86% in discrimination between post-implant group and healthy controls. In the cellular component category, four genes of the five-gene signature except STK10 were related to mitochondrion. The five-gene signature was associated with oxidative phosphorylation, mitochondrial ATP synthesis and apoptosis in biological process analysis. Pathway analysis indicated that a significant enrichment of candidate genes involved in the calcium signaling and glycosphingolipid biosynthesis pathways. The expression changes observed in this study reflect a profound effect of ventricular mechanical dyssynchrony caused by RVA pacing on the transcriptome at an early stage. RVA pacing alters cellular energy metabolism may have association with mitochondrial dynamics.
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PMID:Altered mitochondrial expression genes in patients receiving right ventricular apical pacing. 2717 95