Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.11.8 (
FAST
)
758
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Methods to detect the presence of genetically modified organisms (GMOs) are evolving constantly to comply with legislation and include new transgenic traits developed by biotechnology companies. Since traditional screening methods target only a limited number of markers, not all GM-events can be detected easily. To cover a broader range, a new GMO screening method was developed, based on two real-time PCR reactions, targeting i) six major GM-markers and ii) six GM-events that do not contain these markers. This method used the
FAST
PCR technology and allowed a further reduction in time-to-result. Using a wide array of reference materials and proficiency test samples, the method showed a broad screening capacity and the absolute limit of detection was consistently below
20 copies
, thereby demonstrating the method is fit-for-purpose.
...
PMID:Two FAST multiplex real-time PCR reactions to assess the presence of genetically modified organisms in food. 3037 5
