Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.11.27 (
AMPK
)
6,299
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
NRAGE
has been reported to be overexpressed in cancer cells, especially in lung cancer cells. To determine the role of
NRAGE
in non-small-cell lung cancer cells, we investigated the effects of
NRAGE
on autophagy in non-small-cell lung cancer cells. Human A549 and H1299 cells were transfected with
NRAGE
-specific small-interfering RNA. The Cell Counting Kit-8 and plate clone assay showed that downregulation of
NRAGE
could induce the proliferation in A549 and H1299 cells. In addition, our data suggested that downregulation of
NRAGE
enhances autophagosome formation by immunofluorescence. We found that knockdown of
NRAGE
induced autophagy, together with downregulation of P62 and upregulation of LC3-II protein. Furthermore, to elucidate the mechanism of
NRAGE
in suppressing autophagy, the protein expressions of
AMPK
, Ulk1, and Atg13 were assessed. Collectively, these results demonstrate the effective anti-autophagic of
NRAGE
in non-small-cell lung cancer cells through
AMPK
/Ulk1/Atg13 autophagy signaling pathways. Therefore,
NRAGE
could be used as a potential therapeutic target for lung cancer.
...
PMID:Silencing of NRAGE induces autophagy via AMPK/Ulk1/Atg13 signaling pathway in NSCLC cells. 2863 9
