Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:2.7.11.27 (AMPK)
 6,299 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The development of high performance liquid chromatography method on amylose-based stationary phase (Chiralpak AD) with n-hexane-2-propanol (90:10, v/v) as mobile phase allowed the enantioseparation of non-dansylated and dansylated benzimidazole derivatives, prepared from potent-AMPK activators, to be achieved. Using both fluorescence and ultraviolet detection, limits of detection and quantification were determined. Fluorescence detection seems to be the most appropriate technique since the first enantiomer of dansylated benzimidazole 8 eluted with a limit of quantification of 2.25 nm. The quantification limit was improved 10-fold by fluorescence detection compared with a previous report describing mass spectrometry detection. Linearity and repeatability parameters were validated. These lower limits obtained by fluorescence detection, associated with good resolution observed for the dansylated derivatives, make this chiral methodology convenient for the use of these fluorescent potent-AMPK activators as probes to elucidate their cellular localization and their mechanism of action.
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PMID:Development of HPLC/fluorescence detection method for chiral resolution of dansylated benzimidazoles derivatives. 2387 15

Accumulating evidence reveals the association of 1, 3-dichloro-2-propanol (1, 3-DCP) exposure and lipogenesis. Alliin, the most abundant sulphur compound in garlic, has been demonstrated to exhibit hypoglycemic, antioxidant and anti-inflammatory activities. Here, we showed that alliin attenuated lipogenesis induced by 1,3-DCP and that the reduction was due to activation of the AMPK pathway. HepG2 cells exposed to 1,3-DCP exhibited significant increase of triglyceride(TG) and total cholesterol(TC), and alliin reduced the accumulation. Most importantly, alliin could up-regulate the phosphorylation of AMPK and down-regulate protein and gene expressions of SREBP-1; FAS; SREBP-2;HMGCR in 1,3-DCP-induced HepG2 cells. The results demonstrated that alliin was effective on attenuating 1,3-DCP-induced lipogenesis via activation of the AMPK-SREBPs signaling pathway in HepG2 cells.
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PMID:Alliin attenuates 1, 3-dichloro-2-propanol-induced lipogenesis in HepG2 cells through activation of the AMP-activated protein kinase-dependent pathway. 2930 3