Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.11.26 (
GSK
)
6,788
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
We have been developing a unique system for the downregulation of a gene expression through cutting a specific mRNA by the long form of tRNA 3'-processing endoribonuclease (
tRNase Z
(L)) under the direction of small-guide RNA (sgRNA). However, the efficacy of this system and the involvement of
tRNase Z
(L) in the living cells were not clear. Here we show, by targeting the exogenous luciferase gene, that the efficacy of the sgRNA/
tRNase Z
(L) method can become comparable to that of the RNA interference technology and that the gene silencing is owing to
tRNase Z
(L) directed by sgRNA not owing to a simple antisense effect. We also show that
tRNase Z
(L) together with sgRNA can downregulate expression of the endogenous human genes Bcl-2 and
glycogen synthase kinase-3beta
by degrading their mRNAs in cell culture. Furthermore, we demonstrate that a gene expression in the livers of postnatal mice can be inhibited by an only seven-nucleotide sgRNA. These data suggest that sgRNA might be utilized as therapeutic agents to treat diseases such as cancers and AIDS.
...
PMID:Gene silencing by the tRNA maturase tRNase ZL under the direction of small-guide RNA. 1688 98
