Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.11.26 (
GSK
)
6,788
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Increasing evidence has shown that pseudogenes can widely regulate gene expression. However, little is known about the specific role of
PTENP1
and miR-499-5p in insulin resistance. The relative transcription level of
PTENP1
was examined in db/db mice and high fat diet (HFD)-fed mice by real-time PCR. To explore the effect of
PTENP1
on insulin resistance, adenovirus overexpressing or inhibiting vectors were injected through the tail vein. Bioinformatics predictions and a luciferase reporter assay were used to explore the interaction between
PTENP1
and miR-499-5p. The relative transcription level of
PTENP1
was largely enhanced in db/db mice and HFD-fed mice. Furthermore, the overexpression of
PTENP1
resulted in impaired Akt/
GSK
activation as well as glycogen synthesis, while
PTENP1
inhibition led to the improved activation of Akt/
GSK
and enhanced glycogen contents. More importantly,
PTENP1
could directly bind miR-499-5p, thereby becoming a sink for miR-499-5p.
PTENP1
overexpression results in the impairment of the insulin-signaling pathway and may function as a competing endogenous RNA for miR-499-5p, thereby contributing to insulin resistance.
...
PMID:Pseudogene PTENP1 Functions as a Competing Endogenous RNA (ceRNA) to Regulate PTEN Expression by Sponging miR-499-5p. 2744 20
