Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.11.22 (
cdc2
)
8,319
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Many of the genes that control cyclin-dependent kinase (Cdks) activity have been identified by genetic research using yeast mutants. Suppression analysis and synthetic enhancement analysis are two broad approaches to the identification of genetic interaction networks in yeasts. Here we show, by genetic analyses using a mammalian cell cycle mutant, that mouse
magoh
is involved in Cdk regulation. Magoh, a homolog of the Drosophila
mago
nashi gene product, is a component of the splicing-dependent exon-exon junction complex (EJC). We show that, in addition to ccnb1 and cks2,
magoh
is also a dosage suppressor of the mouse temperature-sensitive
cdc2
mutant, and synthetic enhancement of the
cdc2
ts phenotype by RNA interference (RNAi) of
magoh
is observed in a manner similar to RNAi of cks2. Moreover, the depletion of
magoh
by RNAi causes cold-sensitive defects in the cell cycle transition, and exogenous cks2 expression partially suppresses the defect. Consistent with the genetic evidence,
magoh
RNAi caused defects in the expression of Cdc2 or Cks proteins, and introns of cks genes strongly affected protein expression levels. Thus, these data suggest that mouse Magoh is related to cell cycle regulation.
...
PMID:Genetic analyses using a mouse cell cycle mutant identifies magoh as a novel gene involved in Cdk regulation. 2121 Sep 8
