Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.11.22 (
cdc2
)
8,319
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
RINGO/Speedy proteins are direct activators of Cdk1 and
Cdk2
that have no sequence homology to cyclins. We have characterized the role in cell-cycle progression of a new human member of this protein family referred to as
RINGO C
. We show that siRNA-mediated knockdown of
RINGO C
results in premature mitotic exit with misaligned chromosomes, even in the presence of microtubule poisons. Time-lapse-microscopy experiments suggest that
RINGO C
is involved in the spindle-assembly checkpoint (SAC). Consistent with this idea, RINGO-C-depleted cells show impaired recruitment of the SAC components Mad2, Bub1 and BubR1. As the checkpoint is overridden, cells display defective chromosome segregation, which leads to an increased number of micronuclei and binucleated structures. Intriguingly, we found that
RINGO C
can associate with the mitotic kinase Aurora B, and downregulation of
RINGO C
produces mislocalization of the active form of Aurora B in prometaphase. Taken together, our results indicate a role for
RINGO C
in the mitotic checkpoint, which might be mediated by defective recruitment of SAC components and deregulation of the activity of Aurora kinase B.
...
PMID:RINGO C is required to sustain the spindle-assembly checkpoint. 2060 20
