Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.11.22 (
cdc2
)
8,319
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Temozolomide (TMZ) is an alkylating chemotherapy agent used in the clinical treatment of glioblastoma multiforme (GBM) patients.
Piperine
(
PIP
) is a naturally occurring pungent nitrogenous substance present in the fruits of peppers. We investigated the anti-cancer efficacies of
PIP
alone and in combination with TMZ in GBM cellsusingparameters such as cell proliferation, cellular apoptosis,caspase-8/-9/-3 activities, cell cycle kinetics, wound-healing ability, and loss of mitochondrial membrane potential (MMP). Treatment with
PIP
and alow concentration of
PIP
-TMZ, inhibited cell growth, similar to TMZ.
PIP
-TMZ promoted apoptosis by activation of caspase-8/-9/-3, MMP loss, and inhibition of
in vitro
wound-healing motility. Reverse transcription polymerase chain reaction analysis showed significant inhibition of
Cyclin-dependent kinases
(
CDK
)4/6-cyclin D
and
CDK2-cyclin-E
expression upon treatment with a low concentration
PIP
-TMZ, suggesting an S to G1 arrest. Our findings provide insight into the apoptotic potential of the combination of a low concentration of
PIP
-TMZ, though further
in vivo
study will be needed for its validation.
...
PMID:Piperine synergistically enhances the effect of temozolomide against temozolomide-resistant human glioma cell lines. 3269 71
