Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.11.22 (
cdc2
)
8,319
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Studies on the functional and structural properties of the
cdc2 kinase
, a key cell-cycle regulator, have been possible thanks to the availability of
cdc2
-specific immunoreagents. In an attempt to elucidate the biochemical regulation of the
cdc2 kinase
in more detail, we have raised a series of novel mouse monoclonal antibodies against human recombinant
cdc2
protein. The five Mab reported here can be subclassified into two groups according to their interspecies cross-reactivity and distinct immunoprecipitation patterns. Thus, the target epitopes of Mab
POH
-1,
POH
-2 and
POH
-7 (group 1) appear to be limited to a few mammalian species and the fraction of
cdc2
immunoprecipitable by these Mab from cellular extracts is considerably enhanced by denaturation. In contrast, the
POH
-3 and
POH
-8 (group 2) Mab recognize a denaturation-sensitive epitope on
cdc2
which is present in all tested mammalian species. More importantly, each of the two groups of Mab immunoprecipitate forms of
cdc2
associated with a characteristic set of cellular proteins, none of which appears to be cyclin A or cyclin B. None of the antibodies precipitated a histone-H1 or casein-kinase activity, although an activity which phosphorylated some of the coprecipitated proteins was coprecipitated with the group 2 Mab. These novel Mab did not interfere with the association of
cdc2
with cyclin A in vitro and efficient immunoprecipitation of a panel of
cdc2
mutant proteins suggests that the target epitopes may not involve amino acid residues essential for currently known
cdc2
functions. The results of the present study provide evidence for the existence of additional forms of the
cdc2
protein in exponentially growing human cells, distinct from both the monomeric and the cyclin-bound
cdc2
identified so far.
...
PMID:Distinct forms of human CDC2 identified by novel monoclonal antibodies. 162 47
