Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.11.2 (
PDK1
)
2,238
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
To better understand the effects of synbiotics administered at early stages of embryonic development in poultry, it is necessary to analyze direct effects (meat quality) and the molecular background. The molecular interpretation of poultry meat properties after
in ovo
administration of synbiotics remains to be reported. The purpose of the present study was to analyze the molecular background of meat quality based on gene expression and basic physiological parameters. Eggs were injected with (S1)
Lactobacillus salivarius
with galacto-oligosaccharides or (S2)
Lactobacillus plantarum
with raffinose family oligosaccharides. The pectoral muscle was collected at two time points (day 7 and day 42) and subjected to RNA isolation. Gene expression analysis was performed by RT-qPCR for a panel of eight genes associated with metabolism. The concentration of glucose and hormones (insulin, glucagon, and leptin (S1
p
= 0.04)) was also increased. The obtained results showed that metabolic gene expression in the muscle was more differential due to synbiotic stimulation on day 7 (
FST
in S1
p
= 0.03;
PDK4
in S1
p
= 0.02 and S2
p
= 0.01;
CEBPB
in S1
p
= 0.01 and S2
p =
0.008;
PHKB
in S1
p =
0.01;
PRKAG3
in S1
p =
0.02) than on day 42 (
PDK4
in S1
p =
0.04). On the basis of the results obtained, it can be concluded that
in ovo
stimulation with S1 triggered the most potent and favorable changes in the pectoral muscle gene expression in broiler chickens.
...
PMID:Metabolic Gene Expression in the Muscle and Blood Parameters of Broiler Chickens Stimulated In Ovo with Synbiotics. 3232 87
